Original Article
Effect of temporomandibular joint disc perforation on expression of type Ⅰ collagen in temporomandibular joint disc cells
Mohong Deng, Jie Xu, Hengxing Cai, Wei Fang, Xing Long
Published 2017-05-09
Cite as Chin J Stomatol, 2017, 52(5): 274-277. DOI: 10.3760/cma.j.issn.1002-0098.2017.05.003
Abstract
ObjectiveTo investigate the effect of temporomandibular joint (TMJ) disc perforation on expression of type Ⅰ collagen in TMJ disc cells.
MethodsThe fibroblastic-like cells from the surgical removed TMJ disc tissue (disc perforation or TMJ condyle hyperplasia) were cultured. The cultured cells were identified as fibroblastic-like cells by toluidine blue and immunofluorescence staining. The expression of type Ⅰ collagen was detected with Western blotting and the content of type Ⅰ collagen was examined by enzyme linked immunosorbent assay (ELISA).
ResultsFibroblastic-like cells were cultured from TMJ disc cells and the controls. The collagen-Ⅰ and collagen-Ⅱ were positive in both toluidine blue and immunofluorescence staining. In Western blotting, the expression of typeⅠcollagen in cells from joints with disc perforation was lower than that from normal joints. The content of collagen-Ⅰ was (1.62±0.52) μg/L from controls, and (0.85±0.33) μg/L from disc perforation respectively (P=0.0134).
ConclusionsThe disc cells from TMJ with disc perforation expressed lower type Ⅰ collagen than that from controls, which may be related to the lower content of collagen-Ⅰ in TMJ disc and the formation of TMJ disc perforation.
Key words:
Temporomandibualr joint disc; Disc perforation; Type Ⅰ collagen
Contributor Information
Mohong Deng
2nd Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China
Jie Xu
2nd Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China [Present address: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, China]
Hengxing Cai
2nd Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China
Wei Fang
2nd Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China
Xing Long
2nd Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China
