Lecture
Research progress of CRISPR/Cas biosensors based on different signal amplification strategies
Tian Benshun, Wu Yunjian, Cui Xuxia, Lyu Jingwen, Chen Minghui, Zhu Chuan, Gu Bing
Published 2023-01-06
Cite as Chin J Prev Med, 2023, 57(1): 112-119. DOI: 10.3760/cma.j.cn112150-20220220-00158
Abstract
CRISPR/Cas(the clustered regularly interspaced short palindromic repeats-CRISPR associated)system exists in most bacteria and all archaea. It is an important strategy for bacteria and archaea to resist foreign nucleic acid invasion and use for self-defense. The CRISPR/Cas system is a simple, fast, and specific diagnostic tool, which is widely used in agriculture, industry, animal husbandry, and medicine. This article mainly introduces and discusses recently advantages and limitations of biosensors combining CRISPR/Cas system with fluorescence, visualization and surface enhanced raman related technologies, as well as future research directions.
Key words:
Clustered regularly interspaced short palindromic repeats; Biosensing techniques; Diagnosis; Clinical application; Research progress
Contributor Information
Tian Benshun
College of Medical Technology, Xuzhou Medical University, Xuzhou 221004, China
Division of Laboratory Medicine, Guangdong Provincial People′s Hospital, Guangdong Academy of Medical Sciences,Guangzhou 510080, China
Wu Yunjian
School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China
Cui Xuxia
College of Medical Technology, Xuzhou Medical University, Xuzhou 221004, China
Division of Laboratory Medicine, Guangdong Provincial People′s Hospital, Guangdong Academy of Medical Sciences,Guangzhou 510080, China
Lyu Jingwen
College of Medical Technology, Xuzhou Medical University, Xuzhou 221004, China
Division of Laboratory Medicine, Guangdong Provincial People′s Hospital, Guangdong Academy of Medical Sciences,Guangzhou 510080, China
Chen Minghui
School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China
Zhu Chuan
Department of Laboratory Medicine, Xuzhou Tumor Hospital, Xuzhou 221005, China
Gu Bing
College of Medical Technology, Xuzhou Medical University, Xuzhou 221004, China
Division of Laboratory Medicine, Guangdong Provincial People′s Hospital, Guangdong Academy of Medical Sciences,Guangzhou 510080, China
