Application of multiplex PCR capillary electrophoresis and analysis of distribution characteristics of HPV infection subtypes
Gao Yuan, Jiang Yue, Tie Yanqing, Yu Ding, Zhao Mengchuan, Tan He, Tang Zhipeng, Zhang Wenchao, Feng Zhishan
Abstract
ObjectiveTo analyze the characteristics of human papillomavirus (HPV) infection and the distribution of HPV subtypes in Shijiazhuang, Hebei Province, and to explore the application evaluation of multiple PCR capillary electrophoresis fragment analysis for HPV typing test.
MethodsA population-based cross-sectional study was conducted among 434 women (age range 17 to 74 years old, 260 patients and 174 physical examinations) included from May to August 2022 in Hebei General Hospital. HPV typing was detected by multiple PCR-capillary electrophoresis fragment analysis. Using the multiple fluorescence quantitative PCR kit as a reference, Chi-square test was used to analyze the diagnostic effect of multiple PCR-capillary electrophoresis fragment analysis, and the consistency was analyzed by Kappa value.
ResultsThe total HPV infection rate was 45.85%(199/434), including 35.48% (154/434) of high-risk HPV (HR-HPV), 3.92% (17/434) of low-risk HPV (LR-HPV), 6.45% (28/434) of HR-HPV and LR-HPV mixed infection, 27.88% (121/434) of single type HPV and 17.97% (78/434) of multi type HPV. HPV52 (9.68%, 42/434), HPV16 (6.91%, 30/434), and HPV58 (6.91%, 30/434) are common HPV subtypes. The positive rate of physical examination was 45.40% (79/174), which was slightly lower than that of patients 46.15% (120/260), there was no significant difference (χ2=0.024,P>0.05). The highest infection rate in the 17-30 age group was 54.76% (46/84), and there was no statistical difference among the age groups(χ2=4.123,P>0.05). The sensitivity and specificity of multiplex PCR capillary electrophoresis fragment analysis were 92.96% and 94.04%, respectively, and Kappa value was 0.870, with the multiplex fluorescent quantitative PCR as the reference.
ConclusionHPV infection may appear younger, and the positive rate of HR-HPV infection is the highest, with HPV52, 16, 58 as the main infection subtypes. The detection results of multiplex PCR capillary electrophoresis fragment analysis method are highly consistent with those of multiplex fluorescent quantitative PCR method, which is suitable for HPV DNA typing.
Key words:
Human papillomavirus; Multiplex polymerase chain reaction; Uterine cervical diseases
Contributor Information
Gao Yuan
Department of Laboratory Medicine, Hebei General Hospital, Shijiazhuang 050057, China
Hebei Key Laboratory of Molecular Medicine, Shijiazhuang 050057, China
Hebei Clinical Research Center for Laboratory Medicine, Shijiazhuang 050057, China
Jiang Yue
Department of Public Health, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, China
Tie Yanqing
Department of Laboratory Medicine, Hebei General Hospital, Shijiazhuang 050057, China
Hebei Key Laboratory of Molecular Medicine, Shijiazhuang 050057, China
Hebei Clinical Research Center for Laboratory Medicine, Shijiazhuang 050057, China
Yu Ding
Ningbo Health Gene Technologies Co., Ltd, Ningbo 315042, China
Zhao Mengchuan
Department of Laboratory Medicine, Children′s Hospital of Hebei Province, Shijiazhuang 050031, China
Tan He
Department of Laboratory Medicine, Hebei General Hospital, Shijiazhuang 050057, China
Hebei Key Laboratory of Molecular Medicine, Shijiazhuang 050057, China
Hebei Clinical Research Center for Laboratory Medicine, Shijiazhuang 050057, China
Tang Zhipeng
Department of Laboratory Medicine, Hebei General Hospital, Shijiazhuang 050057, China
Hebei Key Laboratory of Molecular Medicine, Shijiazhuang 050057, China
Hebei Clinical Research Center for Laboratory Medicine, Shijiazhuang 050057, China
Zhang Wenchao
Department of Laboratory Medicine, Children′s Hospital of Hebei Province, Shijiazhuang 050031, China
Feng Zhishan
Department of Laboratory Medicine, Hebei General Hospital, Shijiazhuang 050057, China
Hebei Key Laboratory of Molecular Medicine, Shijiazhuang 050057, China
Hebei Clinical Research Center for Laboratory Medicine, Shijiazhuang 050057, China
