Original Article
To investigate the effect of Astragaloside Ⅳ on high-glucose-induced cardiomyocytes pyroptosis
Qin Yiran, Zhang Zhiqiang, Chen Xueheng, Wei Xiqing, Zhang Jinguo
Published 2023-03-14
Cite as Chin J Geriatr, 2023, 42(3): 341-346. DOI: 10.3760/cma.j.issn.0254-9026.2023.03.018
Abstract
ObjectiveTo investigate the effect of Astragaloside Ⅳ on high glucose-induced cardiomyocyte pyroptosis.
MethodsH9c2 cells were cultured in vitro and divided into control group(5.5 mmol/L glucose), high glucose group(33.3 mmol/L glucose), Astragaloside Ⅳ group(33.3 mmol/L glucose+ 100μmol/L Astragaloside Ⅳ), and NLRP3 inhibitor group(33.3 mmol/L glucose+ 1μmol/L MCC950). Cell counting kit 8(CCK-8)was used to detect the activity of H9c2 cells.Lactate dehydrogenase(LDH)kit was used to detect the content of LDH in cell supernatant.Superoxide anion fluorescent probe(DHE)was used to detect the level of intracellular reactive oxygen species(ROS). Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to detect the mRNA and protein expression levels of pyroptosis-related genes.Immunofluorescence was used to detect the fluorescence intensity of NLRP3.Enzyme-linked immunosorbent assay(ELISA)was used to detect the level of inflammatory factors in cell supernatant.
ResultsWhen the concentration of Astragaloside Ⅳ was 100 μmol/L, it could significantly inhibit the decrease of cardiomyocyte viability induced by high glucose(P<0.01)and reduce LDH release(P<0.01). Compared with the control group, the level of ROS was increased(P<0.01), the mRNA and protein expressions of pyroptosis-related molecules were up-regulated(P<0.01 for all), the fluorescence intensity of NLRP3 was increased(P<0.01), and the levels of inflammatory factors in the cell supernatant were increased in the high glucose group(P<0.01). Compared with the high glucose group, the ROS level was decreased(P<0.01), the mRNA and protein expressions of pyroptosis-related molecules were down-regulated(P<0.05 or P<0.01), the fluorescence intensity of NLRP3 was decreased(P<0.01), and the levels of inflammatory factors in cell supernatant were decreased(P<0.05 or P<0.01)in Astragaloside Ⅳ group and inhibitor group.
ConclusionsAstragaloside Ⅳ plays a protective role in high glucose-induced cardiomyocyte injury by inhibiting NLRP3/Caspase-1 signaling pathway and inhibiting pyroptosis.Moreover, it can improve the anti-inflammatory and antioxidant properties in cell models.
Key words:
Astragaloside Ⅳ; High glucose; Pyroptosis; Cardiomyocytes
Contributor Information
Qin Yiran
Cheeloo College of Medicine, Shandong University, Jinan 250012, China
Zhang Zhiqiang
Clinical Medical School, Jining Medical University, Jining 272067, China
Chen Xueheng
Clinical Medical School, Jining Medical University, Jining 272067, China
Wei Xiqing
Department of Cardiology, Affiliated Hospital of Jining Medical University, Jining 272029, China
Zhang Jinguo
Department of Cardiology, Affiliated Hospital of Jining Medical University, Jining 272029, China
