Radiobiology
Melatonin inhibits UVB-induced melanin synthesis by regulating p53-mediated premature senescence in HaCaT cells
Ma Liping, Liu Qingjie, Tian Yuting, Liu Fang, Tian Mei, Gao Ling
Published 2021-12-25
Cite as Chin J Radiol Med Prot, 2021, 41(12): 920-925. DOI: 10.3760/cma.j.issn.0254-5098.2021.12.007
Abstract
ObjectiveTo explore the mechanism and regulatory effects of melatonin on UVB-induced melanin synthesis in human immortalized keratinocytes (HaCaT), so as to provide a theoretical basis for the skin protection of melatonin.
MethodsHaCaT cells were pretreated with 10-5 mol/L melatonin and then irradiated with 80 mJ/cm2UVB. The melanin content was detected by NaOH assay, the proportion of premature senescence cells was detected by β-galactosidase staining kit, and the protein expression levels of both p53 and tyrosinase (TYR) were detected by Western blot at 72 h after UVB exposure. After 12 h pretreatment of ATM/ATR inhibitor, p53 inhibitor and melatonin, the proportion of premature senescence and the change of melanin content in HaCaT cells were detected at 72 h after 80 mJ/cm 2 UVB irradiation.
ResultsMelatonin inhibited UVB-induced increases of melanin content (t=56.65, 13.39, P<0.05) and TYR expression (t=16.46, P<0.05) in HaCaT cells. Melatonin alleviated UVB-induced premature senescence (t=7.139, P<0.05) and inhibited UVB-induced increase of p53 expression (t=19.08, P<0.05) in HaCaT cells. In addition, ATM/ATR inhibitor, p53 inhibitor and melatonin all inhibited UVB-induced increase of melanin content in HaCaT cells.
ConclusionsMelatonin inhibits TYR-mediated melanin synthesis by regulating p53-related premature senescence in HaCaT cells after UVB irradiation.
Key words:
Melatonin; Melanin synthesis; Premature senescence; p53; TYR
Contributor Information
Ma Liping
Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
Liu Qingjie
Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
Tian Yuting
Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
Liu Fang
Department of Dermatology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Tian Mei
Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
Gao Ling
Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
