Basic Immunology
Water extract of Glycyrrhiza uralensis Fisch. enhanced the activation of RAW264.7 cells through TLR4 signaling pathway
Aipire Adila·, Pengfei Yuan, ·Aibibuli Reziwanguli, Shanshan Cai, Fuchun Zhang, Jinyao Li
Published 2017-05-31
Cite as Chin J Microbiol Immunol, 2017, 37(5): 333-339. DOI: 10.3760/cma.j.issn.0254-5101.2017.05.002
Abstract
ObjectiveTo investigate the effects of water extract of Glycyrrhiza uralensis Fisch. (GUWE) on the activation of RAW264.7 cells and the possible mechanism.
MethodsRAW264.7 cells were treated with GUWE containing different concentrations of polysaccharide (10, 50, 100, 500 μg/ml). Viability of these cells was analyzed by MTT assay. Phagocytic activity and surface molecules expressed on these cells were detected by flow cytometry. Levels of cytokines were analyzed by ELISA. Western blot assay was performed to analyze the activation of key molecules in TLR4 signaling pathway.
ResultsGUWE at the concentration of 500 μg/ml significantly decreased the viability of RAW264.7 cells, but significantly increased the viability of RAW264.7 cells at concentrations of 50 μg/ml and 100 μg/ml. GUWE significantly enhanced the phagocytic activity of RAW264.7 cells as well as the expression of cytokines and costimulatory molecules in a dose-dependent manner. Further analysis indicated that the activation of RAW264.7 cells induced by GUWE was suppressed by TLR4 inhibitor. Moreover, GUWE enhanced the phosphorylation of NF-κB p65 and TLR4 downstream mitogen-activated protein kinases (MAPKs) including p38, extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK).
ConclusionThis study indicates that GUWE promotes the activation of RAW264.7 cells through TLR4 signaling pathway.
Key words:
Water extract of Glycyrrhiza uralensis Fisch.; RAW264.7 cell; Costimulatory molecule; Cytokine; TLR4
Contributor Information
Aipire Adila·
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
Pengfei Yuan
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
·Aibibuli Reziwanguli
Department of Pediatrics, Occupational Disease Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830052, China
Shanshan Cai
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
Fuchun Zhang
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
Jinyao Li
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
