Clinical Immunology
Genetic polymorphisms of TNF-α and IL-6 and their correlations with susceptibility to colorectal cancer among Chinese Han people in Shandong province
Peixiang Xing, Shifeng Kan, Falin Yang, Jinbo Jiang, Yuanquan Si, Ailan Wang
Published 2017-05-31
Cite as Chin J Microbiol Immunol, 2017, 37(5): 369-373. DOI: 10.3760/cma.j.issn.0254-5101.2017.05.007
Abstract
ObjectiveTo study the correlations between genetic polymorphisms of TNF-α as well as IL-6 and susceptibility to colorectal cancer among Chinese Han people in Shandong province.
MethodsSingle nucleotide polymorphisms (SNPs) of TNF-α-238G/A, -308G/A and IL-6-174G/C, -572G/C, -597G/A in 490 patients with colorectal cancer were analyzed by using gene chip. Concentrations of TNF-α and IL-6 in serum samples were measured by ELISA. A case-control study was conducted to analyze the correlations between SNPs of TNF-α -238G/A, -308G/A as well as IL-6 -174G/C, -572G/C, -597G/A and susceptibility to colorectal cancer. Chi-square test or t test was used for statistical analysis. Relative risks were estimated based on the values of odds ratio (OR) and 95% confidence interval (95%CI).
ResultsThe frequency of TNF-α -308AA in patients with colorectal cancer was significantly higher than that in healthy subjects (χ2 =6.15, P<0.05, OR=2.08, 95%CI=1.17-3.71), while the frequency of IL-6 -572CC in patients with colorectal cancer was significantly lower than that in healthy subjects (χ2 =4.97, P<0.05, OR=0.73, 95%CI=0.55-0.96). The frequency of TNF-α -308AA in patients with colon cancer (OR=2.31, 95%CI=1.17-4.55), tubular adenocarcinoma (OR=2.32, 95%CI=1.28-4.21), high (OR=2.05, 95%CI=1.01-4.15) or moderately differentiated adenocarcinoma (OR=5.88, 95%CI=1.79-19.30) was significantly higher than that in healthy subjects. The levels of serum TNF-α in TNF-α -308AA carriers with colorectal cancer were significantly higher than those in TNF-α -308G carriers with colorectal cancer (t=2.13, P<0.05) as well as those in healthy TNF-α -308AA carriers (t=2.13, P<0.05). The levels of serum IL-6 in colorectal cancer group were significantly higher than those in control group (t=6.74, P<0.001).
ConclusionThe SNPs of TNF-α -308 and IL-6 -572 are associated with the occurrence and development of colorectal cancer in Chinese Han people in Shandong province.
Key words:
Colorectal cancer; Cytokine; Single nucleotide polymorphism
Contributor Information
Peixiang Xing
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China
Shifeng Kan
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China
Falin Yang
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China
Jinbo Jiang
Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
Yuanquan Si
Department of Clinical Laboratory, Shandong Provincial Hospital, Jinan 250021, China
Ailan Wang
Department of Clinical Laboratory, Shandong Cancer Hospital, Jinan 250117, China
