Basic Immunology
Immunoreactivity and diagnostic value of Mycobacterium tuberculosis PstS1 and HspX
Li Na, Li Machao, Xiao Tongyang, Yan Yuhan, Li Xiaoqin, Liu Haican, Wan Kanglin
Published 2020-03-31
Cite as Chin J Microbiol Immunol, 2020, 40(3): 192-197. DOI: 10.3760/cma.j.cn112309-20191104-00364
Abstract
ObjectiveTo evaluate the humoral and cellular immunoreactivity of recombinant Mycobacterium tuberculosis (M.tuberculosis) PstS1 and HspX protein antigens in order to provide reference for immunodiagnosis of tuberculosis and screening of candidates for vaccine antigens.
MethodsPurified recombinant M. tuberculosis PstS1 and HspX proteins were obtained using molecular cloning expression and Ni2+ affinity chromatography. Blood samples and epidemiological data of healthy individuals and patients with M. tuberculosis infection were collected. Specific IgG antibodies and IFN-γ-producing antigen-specific T cells were respectively detected by enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot assay (ELISPOT) with the recombinant proteins used as antigens. The humoral and cellular immunoreactivity of the recombinant PstS1 and HspX proteins were assessed with statistical analysis of data.
ResultsBoth the recombinant PstS1 and HspX proteins could induce the secretion of IFN-γ by more specific effector T cells in patient withM. tuberculosis infection, and the differences between the infection and healthy control groups were statistically significant (P<0.05). The specificity and sensitivity of the recombinant PstS1 and HspX as the diagnostic antigens of ELISPOT were 92.11% (35/38) and 65.96% (31/47), and 68.42% (26/38) and 91.49% (43/47), respectively. The two proteins also possessed some humoral immunoreactivity, but statistically significant difference was only observed in the HspX-specific antibody level between the two groups (P<0.05).
ConclusionsBoth the recombinant PstS1 and HspX proteins had good cellular immunoreactivity and were the immunodominant antigens of cellular immunity. They performed well in cellular immunodiagnosis and were good potential candidate antigens for anti-tuberculosis vaccines.
Key words:
Mycobacterium tuberculosis; PstS1; HspX; Immunodominant antigens
Contributor Information
Li Na
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Li Machao
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xiao Tongyang
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Guangdong Key Lab for Diagnosis &
Treatment of Emerging Infectious Diseases, the Third People′s Hospital of Shenzhen, the Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China
Yan Yuhan
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China
Li Xiaoqin
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Department of Clinical Laboratory, the First Hospital of Changsha, Changsha 410005, China
Liu Haican
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wan Kanglin
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
