HIV
HIV-1 genetic characteristics and drug resistance in newly diagnosed population in Baoding city of Hebei Province
Fan Weiguang, Xing Ying, Han Leilei, Su Miaomiao, Meng Juan, Dai Erhei, Shi Penghui, Li Hanping
Published 2022-02-28
Cite as Chin J Microbiol Immunol, 2022, 42(2): 88-93. DOI: 10.3760/cma.j.cn112309-20210814-00272
Abstract
ObjectiveTo investigate the distribution of HIV-1 genotypes and drug resistance in newly diagnosed HIV-1 patients in Baoding in 2020.
MethodsA self-developed method was used to amplify the pol gene sequence of HIV-1, and the sequencing results were analyzed by phylogenetic analysis and compared with the Stanford drug resistance database to determine the HIV-1 subtypes and gene mutations.
ResultsA total of 96 patients with HIV-1 infection were recruited in this study, and 83 pol gene sequences were successfully obtained. In the study population, 88 (91.7%) were male with an average age of 39 years and 54 (56.3%) were married. Most of the patients were infected through sexual contact (95.8%, 92/96), and 75.0% (72/96) were through homosexual transmission. Phylogenetic analysis showed that various HIV-1 subtypes were detected and among them, CRF01_AE (51.8%, 43/83), CRF07_BC (24.1%, 20/83) and B subtype (10.8%, 9/83) were the most epidemic strains. Moreover, the subtypes of newly identified recombinant strains in recent years accounted for 13.3% (11/83). Drug resistance test results showed that the pre-treatment drug resistance rate in newly diagnosed HIV-1 patients was 8.4% (7/83), and the drug resistance rates to protease inhibitor (PIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs) and integrase inhibitors (INIs) were 3.6% (3/83), 1.2% (1/83) and 3.6% (3/83), respectively.
ConclusionsThe HIV-1 subtypes in the newly diagnosed population in Baoding in 2020 were complex and diverse. There were many unique recombinant strains and drug-resistant strains. Therefore, it was necessary to strengthen drug resistance monitoring as well as the prevention and control of HIV-1 infection in this area.
Key words:
HIV-1; Subtypes; Resistance mutations
Contributor Information
Fan Weiguang
Department of Laboratory Medicine, Baoding People′s Hospital, Baoding 071000, China
Xing Ying
Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang Affiliated to Hebei Medicine University, Shijiazhuang 050021, China
Han Leilei
Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
Su Miaomiao
Department of Laboratory Medicine, Baoding People′s Hospital, Baoding 071000, China
Meng Juan
Department of Laboratory Medicine, Baoding People′s Hospital, Baoding 071000, China
Dai Erhei
Department of Laboratory Medicine, the Fifth Hospital of Shijiazhuang Affiliated to Hebei Medicine University, Shijiazhuang 050021, China
Shi Penghui
Department of Laboratory Medicine, Baoding People′s Hospital, Baoding 071000, China
Li Hanping
Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
