Original Article
Isolation and genome-wide evolutionary analysis of a type Ⅲ WU polyomavirus strain
Huang Yiman, Chen Aijun, Wang Chao, Wei Tianli, Hu Zhuang, Cong Shanshan, Tan Jingjing, Yao Lihong, Zheng Lishu
Published 2023-03-31
Cite as Chin J Microbiol Immunol, 2023, 43(3): 182-190. DOI: 10.3760/cma.j.cn112309-20230116-00012
Abstract
ObjectiveTo isolate and culture WU polyomavirus (WUPyV), and to analyze the genome-wide evolutionary patterns, homology and population dynamics.
MethodsReal-time quantitative PCR was used to detect the nasopharyngeal aspirate samples of hospitalized children with respiratory tract infection in Beijing Friendship Hospital during 2020 to 2022. Primary human airway epithelial cells cultured at the air-liquid interface were used to isolate and culture WUPyV. Whole genome sequence of the isolated strain was obtained by Sanger sequencing. For phylogenetic and evolutionary dynamics analysis, the whole genome was compared with the published whole genome sequences in GenBank database.
ResultsThe detection rate of WUPyV was 4.7% (31/659) during 2020 to 2022, and a clinical strain BJ0593 of WUPyV type Ⅲc was successfully isolated. The homology of the whole genome and gene fragments of WUPyV was high. The average evolutionary rate of VP2 gene was about 1.256×10-4 substitution/site every year, and the population dynamics of WUPyV tended to be flat in the last decade.
ConclusionsThis study successfully isolated a clinical WUPyV type Ⅲ strain for the first time, which provided the basis for further investigation on the molecular evolution and pathogenicity of WUPyV.
Key words:
WU polyomavirus; Virus isolation; Human airway epithelial cell; Whole-wide analysis
Contributor Information
Huang Yiman
Key Laboratory of Medical Virology and Viral Diseases, National Health Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Chen Aijun
Key Laboratory of Medical Virology and Viral Diseases, National Health Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Wang Chao
Key Laboratory of Medical Virology and Viral Diseases, National Health Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Wei Tianli
Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
Hu Zhuang
Department of Hygienic Toxicology, School of Public Health, China Medical University, Shenyang 110122, China
Cong Shanshan
Department of Hygienic Toxicology, School of Public Health, China Medical University, Shenyang 110122, China
Tan Jingjing
Department of Hygienic Toxicology, School of Public Health, China Medical University, Shenyang 110122, China
Yao Lihong
Key Laboratory of Medical Virology and Viral Diseases, National Health Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Zheng Lishu
Key Laboratory of Medical Virology and Viral Diseases, National Health Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
