Objective To evaluate the reliability and validity of the Chinese version of the King＇s Health Questionnaire （KHQ） in patients with overactive bladder（OAB）.Methods The original English KHQ was translated into Chinese and linguistically validated following the Cross-cultural adaptation of health-related quality of life measures. Patients recruited randomly from urology clinics were scheduled for two visits with 2 weeks apart, and they were surveyed through the Chinese version of the KHQ. Internal consistency reliability was assessed by Cronbach＇s α test;Test-retest reliability was examined among stable patients using Intraclass correlation coefficient （ICC） and Spearman＇s rank correlation statistical analyses. Content validity was estimated by Spearman＇s rank correlation statistical analyses. A factor analysis was conducted to validate the underlying factor structure of the Chinese version of the KHQ. Results A total of 48 OAB patients who met the criteria participated the study, and 40 patients （7 men, 33 women） completed the questionnaires twice. All the subscales and domains of the KHQ showed high levels of internal consistency （Cronbach＇s a： 0.718-0. 924） , moderate to excellent test-retest reliability （ICC：0.551-0.923,P〈0.01） and acceptable construct validity. The content validity was moderate to excellent except for the Social Limitations domain. Conclusion Psychometric testing supports the reliability and validity of the Chinese version of the KHQ as an OAB-specific measure of HR QOL.