Laboratory Research
Phenotype and genotype of antimicrobial resistance on nasal Staphylococcus aureus isolates from healthy people
Xiaomei Yan, Xiaoxia Tao, Xiaojie Yu, Jun Yan, Jianzhong Zhang
Published 2015-06-10
Cite as Chin J Epidemiol, 2015, 36(6): 639-643. DOI: 10.3760/cma.j.issn.0254-6450.2015.06.022
Abstract
ObjectiveTo investigate the antimicrobial susceptibility and molecular nature related to the resistance on macrolides from nasal Staphylococcus (S.) aureus isolates among healthy people.
MethodsA total of 100 S. aureus isolates collected from 2009 to 2011 were tested for antimicrobial susceptibility by E-test. Double disc test (D-test) was used to detect the inducible clindamycin resistance. All S. aureus isolates were characterized by spa typing. Macrolides resistance genes were detected and compared with isolates that were collected clinically or from the livestock.
ResultsHigh resistance rates on erythromycin or clindamycin was noticed, with 52% and 27%, respectively. Inducible clindamycin resistance was identified in 29 of the 100 (29%) isolates. In total, the 100 isolates were assigned to 35 spa types. The most common spa types were found to be t189, t571, t002, t796, t437, t034 and t701, that accounted for 51.0% of all the isolates. erm (C) (57.7%) and erm (B) (34.6%) were found as the dominant genes in 52 S. aureus isolates from healthy people. On the other hand, erm (A) and erm (C) were identified in 95.0% S. aureus isolates from patients and all the livestock, respectively.
Conclusionerm (C) and erm (B) carrying S. aureus strains were circulating in healthy people and these genes were distributed in different S. aureus clones.
Key words:
Staphylococcus aureus; Antibiotic susceptibility; Macrolide; spa typing
Contributor Information
Xiaomei Yan
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xiaoxia Tao
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xiaojie Yu
Heilongjiang Provincial Center for Disease Control and Prevention
Jun Yan
Heilongjiang Provincial Center for Disease Control and Prevention
Jianzhong Zhang
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
