Clinical Study
Study on the interaction between matrix metalloproteinases gene polymorphism and central obesity in nonalcoholic fatty liver disease
Pengbo Wu, Yongxiang Shu, Ming Li, Hesheng Luo, Guo Zhang, Shiyun Tan
Published 2015-12-10
Cite as Chin J Epidemiol, 2015, 36(12): 1415-1418. DOI: 10.3760/cma.j.issn.0254-6450.2015.12.022
Abstract
ObjectiveTo study whether matrix metalloproteinases-9 (MMP) -1562C/T (rs3918242) and MMP-2-1306C/T (rs243865) were associated with the susceptibility on nonalcoholic fatty liver disease (NAFLD) and the interactions between the two factors and central obesity.
MethodsGenotypes of 545 patients and 636 subjects with NAFLD under control were examined by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). Unconditional logistic regression (ULR) was performed to assess the NAFLD risk. The gene-environment interactions on the risk of NAFLD were explored by generalized multifactor dimensionality reduction (GMDR) and ULR methods.
ResultsResults from the case-control analysis indicated that there was an increased risk of developing NAFLD for MMP-9 rs3918242 (TT/CT) genotype carriers, when compared with the non-carriers (CC) , with OR=1.67, 95%CI: 1.32-2.12, P=0.001; Adjusted OR=1.65, 95% CI: 1.31-2.01 (P=0.008). However, risk reduction of NAFLD was found when MMP-2 rs243865 (TT/CT) genotype carriers compared with the non-carriers (CC) , with OR=0.68, 95% CI: 0.53-0.86, P=0.001; with adjusted OR=0.66, 95% CI: 0.49-0.90 (P=0.007). Data from the GMDR showed that gene-environment interaction among rs3918242 and central obesity on the risk of NAFLD might be significant (P=0.001). By using the ULR method, subjects as central obesity-positive but with genotype CT/TT, appeared having 4.50 (95% CI: 2.78-7.17, P= 0.007) times risk of NAFLD, when compared to the central obesity-negative subjects with genotype CC after adjusting for the covariates.
ConclusionMMP-9 rs3918242, MMP-2 rs243865 were associated with risk of NAFLD while both rs3918242 and central obesity showing synergistic effects on the risk of the NAFLD.
Key words:
Matrix metalloproteinases; Nonalcoholic fatty liver disease; Polymorphism; Central obesity; Interaction
Contributor Information
Pengbo Wu
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China
Yongxiang Shu
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China
Ming Li
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China
Hesheng Luo
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China
Guo Zhang
Department of Gastroenterology, the People's Hospital of Guangxi Zhuang Autonomous Region
Shiyun Tan
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China
