Emerging of Japanese encephalitis virus and Getah virus from specimen of mosquitoes in Inner Mongolia Autonomous Region
Cheng Rui, Nan Xiaowei, Fan Na, Fu Shihong, Si Xiaoyan, Zhang Lin, He Ying, Lei Wenwen, Li Fan, Wang Huanyu, Lu Xiaoqing, Liang Guodong
Abstract
ObjectiveTo investigate the types and distribution of blood-sucking insects and arboviruses in Inner Mongolia autonomous region, and provide basic data for the prevention of arbovirus transmitted disease.
MethodsBlood-sucking insects were collected by lamp trapping method in nature. Mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. Viruses were isolated in cell culture and characterized, using molecular biological methods.
ResultsA total of 24 240 mosquitoes and 17 110 aphids were collected from 2 sites of 5 counties (Flags) in Inner Mongolia in 2014 and during 2017-2018. Among them, Japanese encephalitis virus gene was detected in Culex pipiens pallens, and 4 virus strains isolates which could be stably passaged. The isolates were identified as Getah virus and densonucleosis virus by molecular biology identification. Phylogenetic analysis on the E2 gene of the Getah virus (NMDK1813-1) showed that it belonged to the same evolutionary branch of the Gansu isolates (GS10-2) and having six common amino acid variation sites.
ConclusionsThe emergence of Japanese encephalitis virus and Getah virus from specimen of mosquitoes in Inner Mongolia indicated the new challenges on the prevention and control of arbovirus and related diseases. The results pf this study provided basic data for the prevention and control stretagies of arbovirus transmitted diseases in Inner Mongolia.
Key words:
Japanese encephalitis; Getah virus; Arbovirus
Contributor Information
Cheng Rui
School of Public Health, Qingdao University, Qingdao 266071, China
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Nan Xiaowei
Department of Vector Biological Prevention and Control, Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot 010031, China
Fan Na
School of Public Health, Qingdao University, Qingdao 266071, China
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Fu Shihong
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Si Xiaoyan
Department of Vector Biological Prevention and Control, Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot 010031, China
Zhang Lin
Bayannaoer Center for Disease Control and Prevention, Bayannaoer 015000, China
He Ying
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Lei Wenwen
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Li Fan
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wang Huanyu
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Lu Xiaoqing
School of Public Health, Qingdao University, Qingdao 266071, China
Liang Guodong
Department of Viral Encephalitis, State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
