Laboratory Research
Establishment and application of a multilocus sequence typing assay for Corynebacterium striatum
Wang Chengling, Wang Jiazheng, Liu Zhiguo, Xu Shuai, Zhu Xiong, Li Huan, Wang Xiaoxia, Qiu Xiaotong, Wei Kongjiao, Fan Shihong, Han Lichao, Li Zhenjun
Published 2021-09-10
Cite as Chin J Epidemiol, 2021, 42(9): 1628-1634. DOI: 10.3760/cma.j.cn112338-20210329-00255
Abstract
ObjectiveTo establish a multilocus sequence typing (MLST) assay for Corynebacterium (C.)striatum, explore the population structure and evolution relationship of clinical isolates of C. striatum.
MethodsSeven housekeeping genes (gyrA, gyrB, hsp65, sodA, secA1,rpoB, 16S rRNA) were amplified with PCR by using self-designed specific primers and sequenced. Then, the sequences were assembled with software SeqMan. The gene diversity and gene recombination characteristics were evaluated by using software DnaSP 5.10.01 and Splits tree 4.14.2. The phylogenetic tree and the minimum spanning tree were constructed based on the sequence types (ST) characteristics by using software MEGA 7.0.14 and BioNumerics, respectively. In addition, the genetic evolutionary relationship among STs were analyzed by using software eBURST 3.0.
ResultsThe expected amplification products of seven sites selected in all the test strains were obtained. Splits tree showed that the clustering of all C. striatum strains was consistent, suggesting that gene recombination is the potential driving force for the evolution of C. striatum. All of the 344 C.striatum strains were divided into 72 STs by MLST and 85.7% of the strains formed clonal complexes. CC19 was the predominant clonal complex, whereas ST16 in the clonal complex was detected in the most strains. ST had a certain geographic clustering and a certain correlation with the isolation time.
ConclusionsC. striatum showed high genetic diversity in China and CC19 was the predominant clonal complex. The MLST assay established in this study can be used for the typing of C. striatum, but further improvement is needed.
Key words:
Corynebacterium striatum; Multilocus sequence typing; Housekeeping gene; Sequence type
Contributor Information
Wang Chengling
Department of Medicine, Tibet University, Lhasa 850000, China
Wang Jiazheng
Department of Clinical Laboratory Medicine, The First Affiliated Hospital (Shandong Qianfoshan Hospital) of Shandong First Medical University, Shandong Medicine and Health Key Laboratory of Laboratory Medicine, Ji'nan 250000, China
Liu Zhiguo
State Key Laboratory for Infectious Disease Prevention and Control , National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xu Shuai
State Key Laboratory for Infectious Disease Prevention and Control , National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhu Xiong
Central and Clinical Laboratory of Sanya People's Hospital of Hainan Province, Sanya 572000, China
Li Huan
Central and Clinical Laboratory of Sanya People's Hospital of Hainan Province, Sanya 572000, China
Wang Xiaoxia
Central and Clinical Laboratory of Sanya People's Hospital of Hainan Province, Sanya 572000, China
Qiu Xiaotong
State Key Laboratory for Infectious Disease Prevention and Control , National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wei Kongjiao
Department of Medicine, Tibet University, Lhasa 850000, China
Fan Shihong
Department of Medicine, Tibet University, Lhasa 850000, China
Han Lichao
State Key Laboratory for Infectious Disease Prevention and Control , National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Li Zhenjun
Department of Medicine, Tibet University, Lhasa 850000, China
State Key Laboratory for Infectious Disease Prevention and Control , National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
