Original Article
Molecular detection and typing of human Metapnenmovirus in children with respiratory tract infection
Yu Kou, Xinfen Yu, Jun Li, Xuhui Yang, Yinyan Zou, Jincao Pan, Li Xie, Zhou Sun, Xiaoying Pu, Shuchang Cen
Published 2016-04-30
Cite as Chinese J Exp Clin Virol, 2016, 30(2): 161-165. DOI: 10.3760/cma.j.issn.1003-9279.2016.02.013
Abstract
ObjectiveTo understand the infection status and molecular features of human metapnenmovirus (hMPV) among pediatric outpatients and inpatients.
MethodsAll 2 593 children with respiratory tract infections who visited the sentinel surveillance hospitals from 2011 to 2013 in Hangzhou, , including 1 050 outpatients and 1 016 inpatients, were involved in this study. The throat swabs were collected from influenza-like illness (ILI) in the clinic, and tracheal aspirates from hospitalized children with severe acute respiratory infection (SARI). The specimens were tested using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). The fusion (F) protein gene of hMPV was amplified for sequencing and phylogenetic analysis.
ResultsThe positive rate was 6.62%(111/1 676) in outpatients and 6.32%(58/917) in inpatients, which showed no statistically significant difference by comparing the rates(χ2=0.086, P=0.769). The positive rate in inpatient(6.33%, 43/679)was higher than that in outpatient(2.79%, 19/681) in children <1 year of age(χ2=9.808, P=0.002). The average age of hMPV-positive outpatients (31.33±18.50 months) was elder than inpatients ((31.33±18.50 months). The positive rate in outpatients (13.56%, 59/435)was higher than that in inpatient (8.06%, 20/248) in winter(χ2=4.669, P=0.031). The co-infection rate in inpatients (31.04%, 19/58) was higher than that in outpatients (18.01%, 20/111)(χ2=4.663, P=0.031). Data showed that 3 viral subtypes appeared in outpatient specimens, and the genotype A2 occurred most frequently. The clinical features showed no difference in all subtypes.
ConclusionsThe rates of hMPV infection showed no significant difference between outpatients and inpatients. The average age of outpatients was older than the age of inpatients who infected the hMPV. The positive rate in outpatients was higher than that in inpatient in winter. The main pathogens co-infected with hMPV were different in outpatients and inpatients. The genotype A2 was predominant in the study.
Key words:
Human Metapneumovirus; Children; Influenza-like illness; Severe acute respiratory infection; Molecular typing
Contributor Information
Yu Kou
Microbiology Laboratory, Hangzhou Center for Disease Contorl and Prevention, Hangzhou 310021, China
Xinfen Yu
Jun Li
Xuhui Yang
Yinyan Zou
Jincao Pan
Li Xie
Zhou Sun
Xiaoying Pu
Shuchang Cen
