Original Article
Evaluation of three laboratory routine methods for inactivating influenza virus
Chen Aijun, Zheng Lishu, Yao Lihong, Wang Chao, Zhang Qian, Guo Jianqiang
Published 2020-08-30
Cite as Chinese J Exp Clin Virol, 2020, 34(4): 415-419. DOI: 10.3760/cma.j.cn112866-20200610-00186
Abstract
ObjectiveTo evaluate the effectiveness of three laboratory routine disinfection methods on influenza virus inactivation.
MethodsThe physical method of ultraviolet irradiation and two chemical methods of ethanol and "84 disinfectant" were used to inactivate the influenza virus respectively. After disinfecting the virus samples at different times or concentrations, MDCK cells were inoculated and the inactivation effect of influenza virus was identified by observing cytopathic effect (CPE).
ResultsWhen the influenza virus was irradiated by ultraviolet light for ≥1 min, treated with 75% ethanol for 0.25-32 min, the effective chlorine concentration of "84 disinfectant" was ≥400 mg/L, and the influenza virus was disinfected at room temperature for ≥1 min, no virus infectivity could be detected under the three conditions.
ConclusionsThe condition of inactivating influenza virus by routine disinfection methods in laboratory was explored, which provided reference data for establishing the protection specifications and the emergency treatment plans for influenza virus laboratories.
Key words:
UV radiation; Disinfectant; Inactivation; Influenza virus; Emergency disposal
Contributor Information
Chen Aijun
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China
Zheng Lishu
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China
Yao Lihong
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China
Wang Chao
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China
Zhang Qian
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China
Guo Jianqiang
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China