Original Article
Detection of rotavirus contamination in laboratory and inactivation efficacy of common disinfectant
Shi Wen, Mao Haiyan, Lu Longxi, Sun Yi, Gong Liming, Yan Hao, Zhang Yanjun
Published 2021-10-30
Cite as Chinese J Exp Clin Virol, 2021, 35(5): 505-508. DOI: 10.3760/cma.j.cn112866-20210806-00138
Abstract
ObjectiveTo understand the rotavirus contamination in the environment of microbiology laboratory, identify the key parts prone to contamination in the laboratory, and evaluate the inactivation efficacy of three common disinfectants on rotavirus.
MethodsThe laboratory environment and the surface of personal protective equipment were sampled with virus sampling swab after the experimental operation, and rotavirus nucleic acid was detected by real-time RT-PCR. Rotavirus inactivation experiments were carried out on chlorine containing disinfectant, ethanol and benzalkonium chloride according to the Chinese Technical Standard of Disinfection (2002 version).
ResultsA total of 174 samples were collected and 13 rotavirus nucleic acid positive samples were detected. The overall positive rate was 7.47%. The rotavirus could be inactivated with more than 4.00 log titer reduction, with 500 mg/L sodium hypochlorite disinfectant for 5 minutes, 75% ethanol for 1 minute and 1 500 mg/L benzalkonium chloride for 1 minute.
ConclusionsThere is a risk of rotavirus contamination in the microbiology laboratory. Sodium hypochlorite, ethanol and benzalkonium chloride disinfectants can effectively inactivate rotavirus and can be used for rotavirus decontamination.
Key words:
Rotavirus; Contamination; Disinfectant; Virus inactivation
Contributor Information
Shi Wen
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
Mao Haiyan
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
Lu Longxi
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
Sun Yi
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
Gong Liming
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
Yan Hao
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
Zhang Yanjun
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China