Diagnosis and Treatment of Viral Diseases
Analysis of the clinical value of autoantibodies detection in patients with liver disease
He Chaonan, Yin Shangqi, Li Jiang, Zheng Mei, Meng Huan, Han Ying, Pan Meichen, Chen Jin, Wang Yajie
Published 2021-12-30
Cite as Chinese J Exp Clin Virol, 2021, 35(6): 643-648. DOI: 10.3760/cma.j.cn112866-20210607-0094
Abstract
ObjectiveTo explore the clinical value of autoantibodies in patients with liver disease.
MethodsWe retrospectively analyzed the data of 1 495 outpatients or inpatients with liver disease in Beijing Ditan Hospital of Capital Medical University from August 2020 to April 2021. Indirect immunofluorescence and Western blot were used to detect antinuclear antibody (ANA) and antinuclear antibodies (ANAs).
ResultsANA and ANAs were positive in patients with liver diseases of various etiologies. Among 1 495 patients with liver disease, 494 cases were ANA positive, the positive rate was 33.04%; 573 cases were positive for ANAs, the positive rate was 38.33%. The positive rate of ANA in the immune liver disease group (63.37%) was higher than that in the viral, alcoholic, fatty liver, confounding factors and other liver disease groups, and the difference was statistically significant (P<0.01). The ANA positive rate between the viral, alcoholic, fatty liver, and confounding factor groups was statistically significant (χ2=19.823, P<0.01), the positive rate of ANAs in the immune liver disease group (80.23%) was higher than that in other liver disease groups, and the difference was statistically significant (P<0.05). The antibody titer of immune liver disease group was mainly 1∶1000, and other liver disease etiology groups was mainly 1∶100. The two most common fluorescent karyotypes in liver disease groups of different etiologies are cytoplasmic and nuclear granular types. The most common specific antibody in the immune liver disease group was anti-mitochondrial antibody type 2 (anti-AMA-M2) antibody, the most common anti-Ro-52 antibody in viral, drug-induced, complex etiology, and other etiological groups, and the most common anti-SSA antibody in alcoholic liver disease. Anti-SSA antibody (17.44%), anti-SSB antibody (9.30%), anti-CENP-B antibody (22.09%), anti-Ro-52 antibody (41.28%), anti-AMA-M2 antibody (51.74%) were positive in immune liver disease group, The rate was higher than that of other liver disease etiology groups, and the difference was statistically significant (P<0.01). When the ANA fluorescence karyotype is nuclear granule type, the positive rate of anti-CENP-B antibody, anti-Ro-52 antibody, and anti-AMA-M2 antibody in the immune liver disease group was higher than that in the viral liver disease group (P<0.01), The positive rate of anti-Ro-52 antibody was higher than that of drug-induced liver disease group (P<0.05).
ConclusionsThe ANA titer of autoimmune liver disease was mainly (1∶1 000). ANAs were mainly positive for anti-SSA antibody, anti-SSB antibody, anti-CENP-B antibody, anti-Ro-52 antibody, and anti-AMA-M2 antibody, especially anti-AMA-M2 antibody. When combined with ANA fluorescent karyotype and ANAs for analysis, if the fluorescent karyotype is nuclear particle type, the positive anti-Ro-52 antibody in ANAs is more valuable in distinguishing immunity from viral and drug-induced liver diseases.
Key words:
Antinuclear antibody; Antinuclear antibody profile; Retrospective analysis; Viral liver disease; Autoimmune liver disease
Contributor Information
He Chaonan
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
Yin Shangqi
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
Li Jiang
Department of Clinical Laboratory, Beijing China-Japan Friendship Hospital, Beijing, 100029, China
Zheng Mei
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
Meng Huan
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
Han Ying
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
Pan Meichen
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
Chen Jin
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
Wang Yajie
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing, 100015, China
