Original Article
Analysis of influential factors and clinical value of HIV-1 DNA
Han Ying, Zheng Mei, Han Jinyu, Chen Jin, Meng Huan, Li Yunhui, Liang Jing, Chen Yuan, Wang Yajie
Published 2023-04-30
Cite as Chinese J Exp Clin Virol, 2023, 37(2): 168-172. DOI: 10.3760/cma.j.cn112866-20220922-00197
Abstract
ObjectiveTo explore the influencing factors of HIV-1 DNA and its clinical value.
MethodsThe relationship between HIV-1 DNA and CD4+ T cell count, CD4/CD8, HIV viral load and subtype was analyzed in 304 patients with HIV/AIDS in order to explore the factors affecting HIV-1 DNA and the value of clinical application.
ResultsThere was no statistically significant difference in HIV-1 DNA levels between the different CD4+ T cell level groups (Z=1.194, P>0.05). HIV-1 DNA levels were higher in the CD4/CD8≤0.5 group than in the CD4/CD8>0.5 group (Z=-2.788, P<0.01). HIV-1 DNA levels were higher in the HIV viral load >100 copies/ml group than in the ≤100 copies/ml group (Z=-2.953, P<0.01). HIV-1 DNA levels were higher in those with CD4+ T cell counts ≤200 at diagnosis than in those with CD4+ T cell counts >200 at diagnosis (Z=-2.175, P<0.05). HIV-1 DNA levels were higher in patients with CD4/CD8 ≤0.2 at diagnosis than in those with CD4/CD8 between 0.2 and 0.5, but there was no significant difference between the two groups (Z=-0.893, P>0.05). HIV-1 DNA levels were higher in both groups than in the CD4/CD8≥0.5 group (Z=-2.568, Z=-1.960, P<0.05). Higher HIV-1 DNA levels were found in people with an HIV viral load >100, 000 copies/ml at diagnosis than in people with an HIV viral load ≤100, 000 copies/ml at diagnosis (Z=-3.520, P<0.001). The level of HIV-1 DNA was higher in the CRF01_AE group than in the non-CRF01_AE group, and the difference was statistically significant (Z=-2.848, P<0.01). CD4/CD8 seemed to be a protective factor for HIV-1 DNA>500 copies/ml. (OR=0.214(95%CI: 0.056~0.822, P<0.05)
ConclusionCD4+ T lymphocyte count, CD4/CD8, viral load and subtype are factors that influence HIV-1 DNA levels, while HIV-1 DNA may be informative for immune status assessment and disease progression determination.
Key words:
AIDS; HIV-1 DNA; HIV reservoirs; CD4+ T lymphocytes; HIV viral load
Contributor Information
Han Ying
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Zheng Mei
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Han Jinyu
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Chen Jin
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Meng Huan
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Li Yunhui
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Liang Jing
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Chen Yuan
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
Wang Yajie
Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
