Physics·Technique·Biology
Effect of miR-133b on radiosensitivity of colon cancer SW620 cells by targeting HER-2
Zhao Lei, Wang Tianyu, Wang Xiaomin, Liu Junzhong, Yi Shanyong
Published 2021-04-15
Cite as Chin J Radiat Oncol, 2021, 30(4): 403-406. DOI: 10.3760/cma.j.cn113030-20190902-00359
Abstract
ObjectiveTo evaluate the effect of miR-133b on the apoptosis and radiosensitivity of colon cancer cell line (SW620 cells), and to explore its mechanism.
MethodsSW620 cells were transfected with miR-con (miR-con group), miR-133b mimics (miR-133b group), si-con (si-con group) and si-HER-2(si-HER-2 group) by the liposome method, and then irradiated with 0, 2, 4, 6, 8 Gy. The miR-133b protein expression, HER-2 protein expression, apoptosis, cell survival fraction and cytofluoroactivity in each group were evaluated by qRT-PCR, Western blot, flow cytometry, colony formation assay and dual luciferase reporter gene assay, respectively.
ResultsCompared with the pre-irradiation group, the expression level of miR-133b was significantly down-regulated (P<0.05), whereas that of HER-2 was significantly up-regulated in SW620 cells after irradiation (P<0.05). Overexpression of miR-133b and knockdown of HER-2 remarkably reduced the survival fraction (bothP<0.05), and significantly promoted the apoptosis of SW620 cells (P<0.05). miR-133b could considerably inhibit the fluorescent activity of wild-type HER-2 cells (P<0.05) and negatively regulate the expression of HER-2 protein.
ConclusionmiR-133b can inhibit the survival of colon cancer cells, promote the apoptosis and enhance the sensitivity of radiotherapy probably via the mechanism of targeting HER-2.
Key words:
miR-133b gene; HER-2 gene; Colon cancer cell line; Radiosensitivity
Contributor Information
Zhao Lei
Department of Intervention, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China
Wang Tianyu
Department of Intervention, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China
Wang Xiaomin
Department of Radiation, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China
Liu Junzhong
Department of Intervention, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China
Yi Shanyong
Depatment of Oncology, Zhengzhou Central Hospital Affiliate to Zhengzhou University, Zhengzhou 450000, China
