Basic Research Article
Expression of Toll like receptor in pancreatic cancer cells
Hu Xingchen, Liu Dejun, Liu Wenting
Published 2023-04-28
Cite as Chin J Hepatobiliary Surg, 2023, 29(4): 285-291. DOI: 10.3760/cma.j.cn113884-20220809-00327
Abstract
ObjectiveTo compare the expression difference of Toll like receptor (TLR) and inflammatory factors between pancreatic cancer and normal pancreatic epithelial cells, and explore the correlation between TLR and inflammatory microenvironment.
MethodsNormal pancreatic duct epithelium cells (HPNE) and pancreatic cancer cells (Panc-1 and Mia-PACA-2) were cultured and proteins were obtained. The expression of TLR family protein, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and myeloid differentiation factor 88 (MyD88) were examined by western blot in HPNE, Panc-1 and Mia-PACA-2. The correlations between TLR and inflammation cytokines of pancreatic cancer were analyzed by Pearson correlation analysis.
ResultsCompared with HPNE, the TLR2, TLR3, TLR4, TLR7, TLR8 and TLR9 were highly expressed in Panc-1 and Mia-PACA-2 (all P<0.05). Compared with Panc-1, the expression of TLR2 and TLR4 in Mia-PACA-2 were increased obviously, while the TLR9 expression was mildly decreased (all P<0.05). The expression of IL-6 in HPNE was found less than that in Panc-1 (0.52±0.03 vs. 0.76±0.04) and Mia-PACA-2 (0.52±0.03 vs. 1.12±0.09) with statistical differences (P<0.05). Similarly, the expression of TNF-α was found significantly less than that of Panc-1 cells (0.63±0.04 vs. 0.87±0.06) and Mia-PACA-2 cells (0.63±0.04 vs. 0.95±0.10) with statistical differences (all P<0.05). The expression of IL-6 was found positively correlated with expressions of TLR2 (r=0.964), TLR4 (r=0.968), TLR7 (r=0.844), TLR8 (r=0.668) (all P<0.05), and the expression of TNF-α was found positively correlated with expressions of TLR2 (r=0.805), TLR4 (r=0.893), TLR7 (r=0.847), TLR8 (r=0.780) (all P<0.05). In contrast with HPNE, the expression of MyD88 was found highly expressed in Panc-1 (0.91±0.10 vs. 0.33±0.03) and Mia-PACA-2 (1.14±0.10 vs. 0.33±0.03) (all P<0.001). Compared with Panc-1, the expression of MyD88 in Mia-PACA-2 was obviously increased (1.14±0.10 vs. 0.91±0.10) with statistical difference (P=0.048).
ConclusionThe TLR family may play a critical role in development of pancreatic cancer by regulating the immune microenvironment, and its mechanism may be through upregulating MyD88 which functions as key signal transduction.
Key words:
Toll-like receptors; Pancreatic neoplasms; Inflammation cytokines; Tumor immune microenvironment
Contributor Information
Hu Xingchen
Department of General Surgery, Changzhou NO.2 People’s Hospital, Changzhou 213164, China
Liu Dejun
Department of General Surgery, Changzhou NO.2 People’s Hospital, Changzhou 213164, China
Liu Wenting
Department of Ophthalmology, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China
