Original Article
Analysis of the oxidative products of DNA(8-oxo Gsn) and RNA(8-oxo dGsn) in patients with essential hypertension
Zhang Ruiping, Yu Songlin, Wang Danchen, Zou Yutong, Zhou Weiyan, Cao Yongtong, Qiu Ling
Published 2020-03-11
Cite as Chin J Lab Med, 2020, 43(3): 274-278. DOI: 10.3760/cma.j.issn.1009-9158.2020.03.015
Abstract
ObjectiveAnalysis of the oxidative products of DNA and RNA in patients with hypertension by determination of 8-oxo Gsn and 8-oxo dGsn, respectively.
MethodsThis is an observational study. During August and December, 2018, 139 hypertension patients without other chronic diseases with an average age of (49.6±12.4) years old, and 139 apparently healthy volunteers without hypertension with an average age of (48.5±11.7) years old were recruited. Fasting morning urine were collected. The oxidative products of DNA: 8-oxo Gsn and the products of RNA: 8-oxo dGsn were analyzed using liquid chromatography tandem mass spectrometry. Urine Cr(U-Cr), and other serum biomarkers such as ALT, Cr, UA, Glu, TG, TC were analyzed using automatic biochemical analyzers.
Resultsof 8-oxo Gsn and 8-oxo dGsn were presented as median(quartile). Statistical analysis was performed using SPSS 22.0 and GraphPad Prism 5. Nonparametric test was used to compare the difference of 8-oxo Gsn and 8-oxo dGsn between the hypertension patients and the healthy controls. Results The DNA and RNA oxidative products of 8-oxo Gsn and 8-oxo dGsn in patients with hypertension and their U-Cr-corrected 8-oxo Gsn/U-Cr and 8-oxo dGsn/U-Cr were 14.38(10.39-19.91)ng/ml, 12.97(7.92-18.96)ng/ml, 1.10(0.88-1.38)μg/μmol and 0.96(0.75-1.30) μg/μmol, respectively, which were significantly higher than those in the healthy controls: 11.95(7.52-18.01) ng/ml, 10.12(6.42-15.04) ng/ml, 0.86(0.59-1.21) μg/μmol and 0.72(0.50-1.02) μg/μmol, respectively. After grouped by sex, 8-oxo Gsn in males, 8-oxo Gsn and 8-oxo dGsn in females showed no significant difference between patients with hypertension and healthy controls, however, after U-Cr correction, both males′ and females′ 8-oxo Gsn/U-Cr and 8-oxo dGsn/U-Cr in patients with hypertension were higher than that in the healthy controls.
ConclusionThe oxidative products of DNA and RNA in patients with hypertension were significantly higher than that in healthy controls.
Key words:
Hypertension; DNA; RNA; Guanosine; Deoxyguanosine; Chromatography, liquid; Tandem mass spectrometry
Contributor Information
Zhang Ruiping
Department of Clinical Laboratory, China-Japan Friendship Hospital, Beijing 100730, China
Yu Songlin
Department of Clinical Laboratory, Peking Union Medical College Hospital, Beijing 100029, China
Wang Danchen
Department of Clinical Laboratory, Peking Union Medical College Hospital, Beijing 100029, China
Zou Yutong
Department of Clinical Laboratory, Peking Union Medical College Hospital, Beijing 100029, China
Zhou Weiyan
Department of Clinical Laboratory, National Center of Clinical Laboratory, Beijing Hospital, Beijing 100730, China
Cao Yongtong
Department of Clinical Laboratory, China-Japan Friendship Hospital, Beijing 100730, China
Qiu Ling
Department of Clinical Laboratory, Peking Union Medical College Hospital, Beijing 100029, China
