This consensus was written by Pediatric Disease Laboratory Medicine Expert Committee of Laboratory Medicine Branch of Chinese Medical Doctor Association, who organized the laboratory experts from 40 children′s hospitals and maternal and child health care hospitals in China. In the initial stage, the committee distributed 384 questionnaires to 240 hospitals (86% of tertiary hospitals) in China, collected the information of the daily average blood culture volume, the operation process before blood culture collection, the time of pediatric blood culture collection and the number of blood culture bottles/sets. On the basis of summarizing the questionnaire information and understanding the current situation of children′s blood culture collection in China, the committee reviewed relevant guidelines, standards and literature, consulted clinical microbiology experts, pediatric clinical experts and senior nurses and organized experts to discuss and revise for four times. It improved the clinical indications and clarified standard of blood culture collection in the children. This consensus provides a reference for standardized collection of blood culture collection in children and establishes the foundation for improving the diagnosis and treatment of bloodstream infections in children.