Original Article
Establishment of normal reference interval of lipoprotein-associated phospholipase A2 quality concentration in apparent healthy people aged over 50 years
Lin Yahui, Gong Meiliang, Zhang Peng, Deng Xinli, Yu Jinxing, Zheng Lei, Zhou Zhou, Cong Yulong
Published 2021-01-11
Cite as Chin J Lab Med, 2021, 44(1): 32-38. DOI: 10.3760/cma.j.cn114452-20200823-00683
Abstract
ObjectiveTo investigate the distribution range of lipoprotein-associated phospholipase A2 (Lp-PLA2) levels in apparent healthy people aged over 50 years, establish a corresponding reference interval, and analyze differences between genders and regions.
MethodsA total of 7 897 fasting venous blood samples were collected from 24 centers from apparent healthy people, and 7 084 samples were included in subsequent statistical analysis after excluding samples with missing information and outliers. Four thousand seven hundred and fifty-two samples and 2 332 samples were collected from the northern and southern China, respectively. The average age was (63.8±10.0) years old in this cohort. The average age of 4 029 males and 3 055 females were (64.8±10.3) and (62.6±9.4) years old, respectively. Automatic magnetic particle chemiluminescence method was used to detect Lp-PLA2 levels. The analysis of overall and subgroup reference interval was conducted according to the documents of the People′s Republic of China Health Industry Standard WS/T 402-2012 and CLSI C28-A3.
ResultsThe distribution of Lp-PLA2 levels in apparent healthy people aged over 50 years follows a normal distribution among all participants, male or female population, the average levels were (143.9±52.6), (149.0±53.7) and (137.2±50.3) μg/L, respectively. The 95% reference upper limit is 230.4 μg/L(90%CI 228.6-232.1 μg/L), 237.3 μg/L(90%CI 234.9-239.7 μg/L) and 219.9 μg/L (90%CI 217.3-222.5 μg/L), respectively. The Z value between genders (9.6) was < Z* (16.3) cut-off value, suggesting that the same 95% reference interval can be used for both men and women. The average level of Lp-PLA2 form apparent healthy people aged over 50 years is similar between the northern and southern regions (P=0.12).
ConclusionsPresent study established a unified 95% reference interval of Lp-PLA2 levels for apparent healthy people aged over 50 years through large cohort investigation and analysis. Although the Lp-PLA2 levels is slightly higher in males than in females, the same 95% reference interval can be used for both men and women. The Lp-PLA2 levels obtained from residents living in northern and southern China are similar.
Key words:
Aged; Lipoprotein-associated phospholipase A2; Reference interval
Contributor Information
Lin Yahui
Center of Laboratory Medicine, Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, Chinese Academy of Medical Sciences &
Peking Union Medical College, National Center for Cardiovascular Diseases, Beijing 100037,China
Gong Meiliang
Center of Clinical Laboratory, Second Medical Center of Chinese PLA General Hospital,Beijing 100853,China
Zhang Peng
Laboratory Medicine Department, Nanfang Hospital Southern Medical University,Guangzhou 510515,China
Deng Xinli
Center of Clinical Laboratory, Second Medical Center of Chinese PLA General Hospital,Beijing 100853,China
Yu Jinxing
Center of Laboratory Medicine, Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, Chinese Academy of Medical Sciences &
Peking Union Medical College, National Center for Cardiovascular Diseases, Beijing 100037,China
Zheng Lei
Laboratory Medicine Department, Nanfang Hospital Southern Medical University,Guangzhou 510515,China
Zhou Zhou
Center of Laboratory Medicine, Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, Chinese Academy of Medical Sciences &
Peking Union Medical College, National Center for Cardiovascular Diseases, Beijing 100037,China
Cong Yulong
Center of Clinical Laboratory, Second Medical Center of Chinese PLA General Hospital,Beijing 100853,China
