Flow cytometry is an important tool for the lineage identification and accurate diagnosis of acute leukemia. Many factors lead to the inter-laboratories differences and the difficulty of accurate diagnosis. In Laboratory Medicine Committee of Chinese Association of Integrative Medicine (LMC-CAIM) consensus, we accurately define the antigen expression rate and expression intensity, divide immune marks into basic and optional ones, put forward antibodies and panel selection according to different diseases, make objective evaluation to the existing diagnostic standards, pay attention to accurate gating, correct sellection of control, accurate description of the proportion of tumor cells and antigen expression during data analysis process, and propose that immunophenotypic reports should include written descriptions and color pictures. Finally, we emphasize that as part of the comprehensive diagnosis of acute leukemia, the main task of immunophenotyping is to identify atypical cases, find minor clones, and make objective evaluation.

Acute leukemia; Flow cytometry; Lineage; Consensus