Original Article
Antibacterial effect of a novel sequence type Acinetobacter baumannii isolate on Acinetobacter spp with different drug-resistant phenotypes
Fan Lujuan, Jiu Kangnan, Liu Zhichao
Published 2022-02-19
Cite as Chin J Lab Med, 2022, 45(2): 169-173. DOI: 10.3760/cma.j.cn114452-20211122-00730
Abstract
ObjectivesTo investigate the antibacterial effects of Acinetobacter baumannii on Acinetobacter SPP.
MethodsA Acinetobacter baumannii strain named SL-A.baumannii that can inhibit other Acinetobacter baumannii strains was isolated from the bronchoalveolar lavage fluid of a critically infected patient in the department of neurology, Henan Provincial Hospital on December 10, 2020. To better understand this inhibition effect, the drug resistance, homology and protein fingerprint of the new Acinetobacter baumannii strain, along with 40 other Acinetobacter SPP isolates, were analyzed.
ResultsMultilocus sequence typing (MLST) revealed that a novel allele combination (gltA/gyrB/gdhB/recA/cpn60/gpi/rpoD) 33, 12, 40, 26, 48, 54, 5 was found in SL-A.baumannii, and ST-2442 was assigned by PubMLST (Oxford). Moreover, 36 isolates, including 30 Acinetobacter baumannii strains, 2 Acinetobacter lwoffii strains, 3 Acinetobacter pittii strains and 1 Acinetobacter ursingii strain, were inhibited by SL-A.baumannii, the inhibition rate was 90% (36/40). Among those strains, 18 (50%) were sensitive to all the antibacterial drugs; 16 (44.44%) showed multi-drug resistance(MDR), including 2 pan-drug resistance(PDR) and 11 extensive drug resistance (XDR) strains; the other 2 (5.56%) were only resistant to Trimethoprim/Sulfamethoxazole(SXT).
ConclusionThe new sequence type (ST-2442) Acinetobacter baumannii isolate SL-A.baumannii (PubMLST/Acinetobacter baumannii isolates database/id: 6942) had shown a significantinhibitory effect on Acinetobacter SPP with different drug-resistant phenotypes.
Key words:
Acinetobacter baumannii; Inhibition; Acinetobacter spp; MLST
Contributor Information
Fan Lujuan
Department of Clinical Laboratory, the First affiliated Hospital of Zhengzhou University, Key Clinical Laboratory of Henan Province, Zhengzhou 450052, China
Jiu Kangnan
Department of Clinical Laboratory, the First affiliated Hospital of Zhengzhou University, Key Clinical Laboratory of Henan Province, Zhengzhou 450052, China
Liu Zhichao
Department of Clinical Laboratory, the First affiliated Hospital of Zhengzhou University, Key Clinical Laboratory of Henan Province, Zhengzhou 450052, China