Original Article
Genomic epidemiology analysis of Enterobacter hormaechei subsp. hoffmannii
Xiang Guoxiu, Ma Xingyan, Cai Yimei, Yu Xuegao, Tan Pinghai, Xu Caixia, Huang Bin
Published 2022-09-19
Cite as Chin J Lab Med, 2022, 45(9): 943-949. DOI: 10.3760/cma.j.cn114452-20220721-00428
Abstract
ObjectivesTo investigate the genetic characteristics of the blaNDM-1-carrying plasmid of the multidrug resistant Enterobacter hormaechei subsp. hoffmannii clinical isolate C37, and constructing a phylogenetic tree of the 66 publicly available genomes of the Enterobacter hormaechei subsp. hoffmannii to explore its global epidemic situation.
MethodsCarbapenem-resistant Enterobacter cloacae complex (CRECC) strains isolated from the First Affiliated Hospital of Sun Yat-sen University from August 2014 to August 2021 were collected. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rDNA Sanger sequencing were used for species identification. Micro broth dilution method was used for antibacterial susceptibility test. The polymerase chain reaction (PCR) was used to detect the β-lactamase resistance gene and plasmid-mediated quinolone resistance (PMQR) gene carried by the C37 strain. The conjugation experiment was used to confirm the conjugative metastasis of the resistance genes in C37 strain. Whole genome of the C37 strain was sequenced. Core genome was extracted and the phylogenetic analysis of 66 publicly available Enterobacter hormaechei subsp. hoffmannii was performed.
ResultsEnterobacter hormaechei subsp. hoffmannii C37 strain is resistant to third-generation cephalosporins, carbapenems, aminoglycosides and quinolones, and carries blaACT-5, blaNDM-1, qnrA1, aac(6′)-Ib-cr, oqxAB, fosA, dfrA15 and other resistance genes, as well as IncX3, IncX4, IncFIB and IncFII plasmids. Multilocus sequence typing (MLST) analysis showed that C37 strain belongs to the ST78 type of Enterobacter cloacae complex (ECC).
ConclusionsST78 type Enterobacter hormaechei subsp. hoffmannii is closely related to the spread of carbapenem resistance genes. It is a potential high-risk clone to spread carbapenem resistance genes. The prevalence and trends of ST78 type Enterobacter hormaechei subsp. Hoffmannii should be monitored.
Key words:
Drug resistance, microbial; Enterobacter hormaechei subsp. hoffmannii; Enterobacter cloacae; carbapenemase; Molecular epidemiology
Contributor Information
Xiang Guoxiu
Department of Laboratory Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
Ma Xingyan
Institute of Microbiology, Osaka University, Osaka 530-0000, Japan
Cai Yimei
Department of Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China
Yu Xuegao
Department of Laboratory Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
Tan Pinghai
Department of Hematology, The Second Affiliated Hospital of Southern Medical University (Zhujiang Hospital), Guangzhou 510200, China
Xu Caixia
Translational Medicine Research Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
Huang Bin
Department of Laboratory Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
