At present, the population of baldness were increasing and become more and more younger, the baldness not only affected the appearance, but also causes severe psychological stress on the patients, which seriously affects the quality of patient’s life. In recent years, cell conditioned medium was widely used in wound repair, hair regeneration and other medical fields. This paper reviewed the research on the promotion of hair regeneration by commonly used cell conditioned media, including mesenchymal stem cell conditioned medium, keratinocyte and its stem cell conditioned medium, and low generation dermal papilla cell conditioned medium.