The effect of new therapy of 20 AA compound amino acid plus high dose of vitamin B6 on the expression of coagulation factor genes in rat model of trauma-induced coagulopathy
Ying Chu, Xuwen Zheng, Qing Bao, Wenming Shen, Zheng Liu, Peigen Zhou, Ying Li, Xiaoxing Bian, Qinmei Huang, Huaping Liang, Maoxing Yue
Published 2016-05-10
Cite as Chin J Emerg Med, 2016, 25(5): 586-591. DOI: 10.3760/cma.j.issn.1671-0282.2016.05.010
Abstract
ObjectiveTo investigate the effect of new therapy of 20 AA compound amino acid injection plus high-dose vitamin B6 on the gene expression of coagulation factors in liver of rat with trauma induced coagulophathy(TIC) model.
MethodsThirty male SD rats were randomly(random number) divided into normal group, sham group, control group and new therapy group, and then control group and new therapy group were divided into four sub-groups (4 h, 6 h, 12 h and 24 h after injury, n=3 in each group). The multiple trauma model of rat was established by using Feeney's free fall epidural impact method, plus bilateral femoral fractures and bleeding. According to above intervals, the total RNA was isolated from liver tissue by Trizol method, the mRNA expression level of coagulation factor genes (F Ⅱ, F Ⅶ, FⅨ, F Ⅹ, F Ⅺ, F Ⅻ, F ⅩⅢ A subunit) were determined by real-time PCR method.
ResultsCompared with normal group, except for F Ⅺ and ⅩⅢ A subunit, the expressions of coagulation factor genes in control group and new therapy group were up-regulated significantly from 4 h after injury, but the expressions were down-regulated with time prolonged. Compared with control group, the expression was higher in the new therapy group(P<0.05).
ConclusionsThe new therapy can improve the coagulation function by enhancing the expression of the coagulation factor genes in the liver.
Key words:
Trauma-induced coagulopathy; 20 AA compound amino acid injection; Vitamin B6; Coagulation factor; Gene expression
Contributor Information
Ying Chu
Central Lab, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Xuwen Zheng
Department of Emergency Medicine, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Qing Bao
Department of Neurosurgery, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Wenming Shen
Department of Emergency Medicine, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Zheng Liu
Research Institute of Surgery, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Peigen Zhou
Department of Emergency Medicine, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Ying Li
Department of Emergency Medicine, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Xiaoxing Bian
Department of Neurosurgery, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Qinmei Huang
Department of Emergency Medicine, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
Huaping Liang
Research Institute of Surgery, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Maoxing Yue
Department of Emergency Medicine, Affiliated Wujin Hospital, Jiangsu University, Changzhou 213002, China
