Objective To evaluate the clinical therapeutic effects of Vbeam 595 nm pulsed dye laser in114 cases of port wine stains.Methods 114 patients with port wine stains were treated with Vbeam 595 nm pulsed dye laser with 3～10 ms pulse width and 7mm spot size.The energy fluence used varied from 10 to 15 J/cm2.Dynamic cooling device(DCD)was used to protect epiderm,with DCD spray 30～40 ms and DCD delay 10～30 ms.The interval of therapy was 4～8 weeks.The results were graded on basis of cleaning and fading as follows:grade Ⅰ(＞75%),gradeⅡ(50%to 74%),grade Ⅲ(25%to 49%),and grade Ⅳ(＜25%).Results 114 patients completed the therapy.The number of treatments ranged from 1 to 10(mean 5.9).The results evaluated as grades Ⅰ,Ⅱ,Ⅲ and Ⅳ were 74 patients(64.9%),29 patients(25.4%),9 patients(7.9%)and 2 patients(1.8%),respectively.The therapeutic effect was analysed according to number of treatments.the ages of patients,site and color of the lesions.It was found that the therapeutic effect was prominent in patients of younger age,with pink lesions located on the neck and face.No recurrence and scar happened after 6 months to 2 years follow-up.Conclnsions Vbeam 595nm pulsed dye laser is a safe and effective method for the treatent of port wine stains,with less complications.