Objectives To explore the pathogenic spectrum of hand,foot and mouth disease in Beijing and to analyse the genetic characteristics of the VP4 region of the predominant type of non-EV71,nonCoxA16 enteroviruses.Methods 45 HFMD specimens tested positive for enterovirus and negative for EV71and CoxA16 were collected from April to December of 2009.The vires RNA was extracted,and the VP4 sequence was amplified by nested RT-PCR.The PCR positive products were sequenced and the enterovirus type was identified by sequence Blast.The sequence and phylogenetic analysis of the predominant rirus type Was performed.Results 32 out of 45(71%)specimens were tested positive by nested RT-PCR,among which 26 PCR products were successfully sequenced.And the types of the 26 enterovirus strains were identified by sequence Blast:11 CoxA10,5 CoxA5,2 CoxA12,2CoxA4,1CoxA2,1CoxA6,1CoxA9,1CoxA16,1 CoxB5 and 1 Echo11.CoxA10 accounted for 44%in typed non-EV71,non-CoxA16 enteroviruses,and was the predominant type.The 11 CoxA10 strains shared the nucleotide identities of 82%-99%and the amino acid identities of 96%-100%with each other in VP4 region.Phylogenetic analysis showed that 10 CoxA10strains were clustered with Guangdong CoxA10 isolates and one clustered with Japan isolates.Conclusions Hand,foot and mouth disease in Beijing was concerned with diverse enteroviruses,and CoxA10 was the predominant type among non-EV71,non-CoxA16 enteroviruses;The CoxA10 strains isolated in Beijing could be clustered into two genotypes.