Original Article
The characteristics of sapovirus infection among patients with diarrhea in Shenzhen in 2016
Hailong Zhang, Yuhui Ye, Yaqing He, Yanjie Wu, Xiangjie Yao, Long Chen, Hong Yang
Published 2019-02-25
Cite as Int J Virol, 2019, 26(1): 31-34. DOI: 10.3760/cma.j.issn.1673-4092.2019.01.09
Abstract
ObjectiveTo investigate the characteristics of sapovirus (SaV) infection among patients with diarrhea in Shenzhen.
MethodsFrom January, 2016 to December, 2016, a total of 838 stool specimens were collected from patients with suspected viral diarrhea. The reverse transcription polymerase chain reaction (RT-PCR) was used to detect the nucleic acid of SaV. The positive PCR products were sequenced and the phylogenetic analysis was performed. The epidemiological data of the cases were analyzed.
ResultsNine samples were found to be positive of SaV, with a positive rate of 1.07% (9/838). There was no significant difference (x2=3.79, P>0.05) in the detection rates between male (1.68%, 8/477) and female (0.55%, 1/361). The detection rates were 0.79% (3/381), 5.00% (1/20), and 1.36% (5/367) among patients in 0-2, 15-19 and ≥20 years old groups respectively. No positive sample was found among patients aged 3-14 years old. The detection rates were 0.99% (2/202) in spring, 1.15% (4/347) in summer, 1.35% (3/222) in autumn, respectively. No positive sample was found in winter. Phylogenetic analysis showed that SaV GI.2 type was the predominant strain in Shenzhen, and SaV GI.1 type was also detected.
ConclusionsSaV was one of the major pathogens of viral diarrhea in Shenzhen, and GI.2 type was the predominant strain.
Key words:
Diarrhea; Sapovirus; Epidemiological study; Genotype
Contributor Information
Hailong Zhang
Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
Yuhui Ye
Health Protection Department, Shenzhen Hospital of Beijing University, Shenzhen 518036, China
Yaqing He
Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
Yanjie Wu
Health Protection Department, Shenzhen Hospital of Beijing University, Shenzhen 518036, China
Xiangjie Yao
Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
Long Chen
Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
Hong Yang
Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
