Prevention and Control of Rotavirus Gastroenteritis
Disinfection effect of heat and ultraviolet on rotavirus
Li Boyang, Li Huiying, Zhang Qing, Wang Mengxuan, Sun Xiaoman, Kong Xiangyu, Wang Hong, Li Dandi, Pang Lili, Pei Yinhui
Published 2021-04-25
Cite as Int J Virol, 2021, 28(2): 108-110. DOI: 10.3760/cma.j.issn.1673-4092.2021.02.005
Abstract
ObjectiveTo examine the efficacy of heat and ultraviolet (UV) radiation in disinfecting rotavirus (RV), so as to provide technical basis for environmental disinfection of RV.
MethodsThe disinfection effect on RV was calculated by the method of median tissue culture infective dose (TCID50) using the change of nucleic acid titer after treatment with heat or UV.
ResultsThere was no significant change in viral activity of RV at 37 ℃ for 24 h. The disinfection effect could be achieved when RV was treated at 56 ℃ and above for 10 min. Radiation of 7 min at the distance of 15 cm or 30 cm from the UV light source could disinfect the RV, and treatment for 10 min at the 15 cm distance or 15 min at 30 cm could inactivate RV. Disinfection could be achieved by treatment for 30 min at a distance of 1 m from the UV light source or 60 min at a distance of 2 m.
ConclusionsRV can be effectively disinfected by 56 ℃ or above, or by close to UV radiation. It is recommended to prolong the disinfection time for long distance from the UV light source. This provides a reference method for disinfecting and inactivating RV.
Key words:
Rotavirus; Physical disinfection; Ultraviolet ray; Heating
Contributor Information
Li Boyang
Hebei Key Laboratory for Chronic Diseases, College of Elementary Medicine, North China University of Science and Technology, Tangshan 063000, China
Li Huiying
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhang Qing
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wang Mengxuan
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Sun Xiaoman
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Kong Xiangyu
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wang Hong
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Li Dandi
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Pang Lili
National Health Commission Key Laboratory of Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Pei Yinhui
Hebei Key Laboratory for Chronic Diseases, College of Elementary Medicine, North China University of Science and Technology, Tangshan 063000, China
