Prevention and Control of Mpox
Genetic characteristics of 181 cases of mpox virus in Beijing in 2023
Li Fu, Jin Haoyuan, Sun Yulan, Zhang Xin, Li Weihong, Yang Yang, Dou Xiangfeng, Ren Zhenyong, Li Dan, Li Jia, Zhang Daitao, Wang Quanyi
Published 2024-04-25
Cite as Int J Virol, 2024, 31(2): 100-103. DOI: 10.3760/cma.j.issn.1673-4092.2024.02.003
Abstract
ObjectiveTo analyze the genomic variations and genetic evolution characteristics of the mpox virus identified from May to September 2023 in Beijing, so as to provide the basis for development of mpox prevention and control measures.
MethodsSamples of throat swabs, skin lesion swabs, and anal swabs were collected from mpox cases. The amplicon sequencing method was use for viral genomes sequencing. The sequences were aligned and analyzed for variation. A phylogenetic tree and a haplotype network were constructed.
ResultsA total of 181 high-quality genome sequences of the mpox virus were obtained and 193 nucleotide mutation sites were identified. Among the mutation sites, 80.8% were driven by the APOBEC3 enzyme. Phylogenetical analysis results showed that the viruses belonged to the C.1 branch of the West African lineage and could be further divided into four different clusters. The haplotype analysis showed that 181 mpox virus genomes can be divided into 108 haplotypes.
ConclusionsThe current circulating mpox virus in Beijing is composed of multiple sources of importation along with continuous transmission and evolution of local mpox virus strains. It is necessary to continue genomic surveillance and research to evaluate the genetic variation of local viruses.
Key words:
Mpox virus; Genome; Phylogenesis; Variation; Haplotype
Contributor Information
Li Fu
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Jin Haoyuan
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Sun Yulan
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Zhang Xin
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Li Weihong
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Yang Yang
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Dou Xiangfeng
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Ren Zhenyong
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Li Dan
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Li Jia
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Zhang Daitao
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
Wang Quanyi
Center Office, Beijing Center for Disease Prevention and Control, Beijing 100013, China
