Experimental and Prescriptions of Chinese Medicine
Research on the quality of Centella asiatica collected in different months based on fingerprint and multi-components determination
Zou Tao, Fang Huilong, Wang Junjie, Liu Qing, Liu Siyu, Zhang Yuanfang, Xie Pengfei, Huang Jianhua
Published 2022-08-30
Cite as Int J Trad Chin Med, 2022, 44(8): 910-916. DOI: 10.3760/cma.j.cn115398-20211126-00330
Abstract
ObjectiveTo establish the HPLC fingerprint of Centellae herba and determine the content of asiaticoside, madecassic acid and asiaticoside B simultaneously; To compare the quality differences of Centellae herba collected in different months.
MethodsThe chromatographic condition was a Shimadzu InertSustain C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile and 2 mmol/L beta cyclodextrin in gradient elution at the flow rate of 0.8 ml/min. The detection wavelength was 204 nm, and the column temperature was 30 ℃. The different Centellae herba materials of collected in 2-12 months from Chenzhou were studied by the similarity evaluation combined with cluster analysis, principal component analysis and the three contents determination.
ResultsThe HPLC fingerprint of Centellae herba was established and 9 common peaks were designated. The eleven samples were different, which can be aggregated into 4 categories and the quality of Centellae herba collected in July was the best.
ConclusionThe established fingerprint and multi-components quantitative method are stable and reliable, which can provide a reference for the quality control and the utilization of Centellae herba resource.
Key words:
Centellae herba; Fingerprint; Chemical pattern recognition; Assay (TCD); Quality control
Contributor Information
Zou Tao
College of Pharmacy, Xiangnan University, Chenzhou 423000, China
Fang Huilong
Basic Medical School, Xiangnan University, Chenzhou 423000, China
Wang Junjie
College of Pharmacy, Xiangnan University, Chenzhou 423000, China
Liu Qing
College of Pharmacy, Xiangnan University, Chenzhou 423000, China
Liu Siyu
College of Pharmacy, Xiangnan University, Chenzhou 423000, China
Zhang Yuanfang
College of Pharmacy, Xiangnan University, Chenzhou 423000, China
Xie Pengfei
Department of Pharmacy, the First People's Hospital of Chenzhou, Chenzhou 423000, China
Huang Jianhua
College of Pharmacy, Xiangnan University, Chenzhou 423000, China
