Experimental Research
Assessment of skeletal muscle early myopathy with contrast-enhanced ultrasound in rabbit model of diabetes mellitus
Weikun Zheng, Guorong Lyu, Zhenhong Xu, Yaya Li, Jiaohong Chen
Published 2019-03-25
Cite as Chin J Ultrasonogr, 2019, 28(3): 267-271. DOI: 10.3760/cma.j.issn.1004-4477.2019.03.015
Abstract
ObjectiveTo evaluate early skeletal muscle myopathy with contrast-enhanced ultrasound (CEUS) in diabetic rabbits.
MethodsAlloxan (100 mg/kg) was given intravenously to 11 New Zealand white rabbits. Another 5 age-matched normal rabbits were used as controls.CEUS was performed at baseline and 1, 2, 3 months after the establishment of diabetes, respectively. Then, skeletal muscle samples were obtained for pathological observation. Additionally, the diabetic rabbits were divided into 3 groups according to their pathologic findings: mild, moderate, and severe myopathy group. Micro-perfusion parameters, including artery-to-vein transit time (A-VTT), muscle tissue mean transit time (M-MTT) and muscle tissue peak intensity (M-PI) were calculated.
ResultsThe diabetic rabbits exhibited a lower body weight increase and a decrease of muscle thickness.Plasma levels of triglyceride, cholesterol and creatinine were significantly higher in diabetic group than those in control group (P<0.05). The A-VTT and M-MTT values of the diabetic rabbits significantly increased over time (all P<0.05), whereas M-PI value significantly decreased compared with that in control group (P=0.013). Besides, the A-VTT value was significantly higher in severe myopathy group than that in mild group (P=0.001). The M-MTT values in both moderate and severe groups were significantly higher when compared to that in mild group (all P<0.05). The A-VTT and M-MTT values were correlated with the severity of diabetic myopathy(ρ=0.898, P<0.001; ρ=0.527, P=0.01).
ConclusionsDiabetic rabbits have defective skeletal muscle micro-perfusion in the early stage. CEUS can quantify impaire muscle microcirculation, and is a valuable tool for assessment of diabetic myopathy.
Key words:
Contrast-enhanced ultrasound; Diabetes mellitus; Myopathy; Microcirculation
Contributor Information
Weikun Zheng
Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
Department of Ultrasound, Xiamen Children′s Hospital, Xiamen 361000, China
Guorong Lyu
Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
Collaborative Innovation Center of Maternal and Child Health Service Technology, Quanzhou Medical College, Quanzhou 361000, China
Zhenhong Xu
Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
Yaya Li
Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
Department of Ultrasound, Xiamen Children′s Hospital, Xiamen 361000, China
Jiaohong Chen
Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
