The resection of bone tumor commonly leads to massive bone defects with diverse morphology. Endoprosthesis with corresponding shape of bone defects in specific patients could be hardly manufactured via conventional technique. Furthermore, given that the endoprosthesis-bone integration determines the long-term outcomes of the reconstruction; new technique which could be able to improve the integration has become one of the research hotspots in reconstruction after bone tumor resection. Thus, novel manufacturing technique is urgently required. Endoprosthesis manufactured via 3D printing technique can match the shape of the bone defects after bone tumor resection precisely. Moreover, the structure on the bone contacting surfaces processed by 3D printing technique has been demonstrated to be able to improve the osseointegration. Therefore, 3D printing technique can serve as an ideal manufacturing method for endoprosthesis reconstructing the bone defects after bone tumor resection. Currently, 3D printing endoprosthesis has been widely used in reconstruction of bone defects after bone tumor resection, especially on special anatomic locations such as pelvis, shoulder, long bone diaphysis, ankle, elbow, wrist, sacrum and spine. Using 3D printing endoprosthesis for reconstruction after pelvic, sacral and spinal tumor resection has been used in case-series studies, which demonstrated satisfied reconstructive outcomes without increasing the surgical risk. However, the longer manufacturing duration of 3D printing prosthesis customized for specific bone defects could limit its application in patients with tumor who has to be operated as soon as possible.