Animal Model
Establishment of rat model of colectomy and anastomosis
Liu Ye, Huang Jinjian, Jiang Yungang, Xu Ziyan, Wu Xiuwen, Ren Jianan
Published 2021-02-08
Cite as Chin J Exp Surg, 2021, 38(2): 371-374. DOI: 10.3760/cma.j.cn421213-20201204-00888
Abstract
ObjectiveTo establish the rats model of colectomy and anastomosis.
MethodsFrom September 2019 to December 2019, 12 male Sprague-Dawley rats provided by the Comparative Medicine Department of the General Hospital of the Eastern Theater Command were divided into two groups randomly: experimental group and control group. There were 9 sprague-dawley (SD) rats in the experimental group, and the colon was cut and sutured. The patients in the control group only underwent exploratory laparotomy. The operation time was recorded and body weight changes were monitored. X-ray examination was done to observe the conditions of intestinal anastomosis at the 7th day after the operation. At the 8th day, the laparotomy was performed again to investigate the morphological changes inside the rat abdominal cavity. The burst pressure of anastomotic stoma was measured. The histological changes of the anastomosis were further detected by collecting the anastomotic and surrounding tissues.T test was used for counting data.
ResultsThe average operation time was 22 min and the survival rate was 89%. The X-ray examination revealed the intestinal canal had no obstruction. The results of laparotomy demonstrated there was slight adhesion at the anastomotic site and the adhesion could be separated. The formation of vascularized tissue blood vessel could be found. Hematoxylin and eosin (HE) staining showed the formation of new granulation tissue and small angiogenesis at the anastomotic site. No colonic epithelium or colorectal glands were found. Masson staining revealed deposition of collagen fibers.
ConclusionThe rat model of colectomy can be constructed via this protocol, which provides an important tool for exploring the healing mechanism of anastomosis after colectomy.
Key words:
Colectomy; Model, animal
Contributor Information
Liu Ye
The Affiliated Second Clinical Hospital, Medical School of Southeast University (General Hospital of Eastern Theater Command), Research Institute of General Surgery PLA, Laboratory for Trauma and Surgical Infections, Nanjing 210000, China
Huang Jinjian
The Affiliated Second Clinical Hospital, Medical School of Southeast University (General Hospital of Eastern Theater Command), Research Institute of General Surgery PLA, Laboratory for Trauma and Surgical Infections, Nanjing 210000, China
Jiang Yungang
The Affiliated Second Clinical Hospital, Medical School of Southeast University (General Hospital of Eastern Theater Command), Research Institute of General Surgery PLA, Laboratory for Trauma and Surgical Infections, Nanjing 210000, China
Xu Ziyan
The Affiliated Second Clinical Hospital, Medical School of Southeast University (General Hospital of Eastern Theater Command), Research Institute of General Surgery PLA, Laboratory for Trauma and Surgical Infections, Nanjing 210000, China
Wu Xiuwen
The Affiliated Second Clinical Hospital, Medical School of Southeast University (General Hospital of Eastern Theater Command), Research Institute of General Surgery PLA, Laboratory for Trauma and Surgical Infections, Nanjing 210000, China
Ren Jianan
The Affiliated Second Clinical Hospital, Medical School of Southeast University (General Hospital of Eastern Theater Command), Research Institute of General Surgery PLA, Laboratory for Trauma and Surgical Infections, Nanjing 210000, China
