Basic Research
Isoflurane anesthesia regulates transcription of RORα and Rev-erbα genes and their circadian rhythm changes in the hippocampus and cerebral cortex of mice
Chen Tianyun, Du Ying, Fang Xin, Xing Fang, Ji Qing
Published 2021-06-15
Cite as Chin J Neuromed, 2021, 20(6): 559-563. DOI: 10.3760/cma.j.cn115354-20210107-00015
Abstract
ObjectiveTo investigate the effects of isoflurane anesthesia on the transcription levels of clock genes RORα and Rev-erbα and their circadian rhythm changes in the hippocampus and cerebral cortex of mice.
MethodsThirty-six 7-week-old male C57BL/6J mice were divided into control group and isoflurane anesthesia group (n=18) according to random number table method. They were subjected to 12 h light/12 h dark environment for 1 week. Mice in the isoflurane anesthesia group were anesthetised with 1.2% isoflurane at 22:00 pm on the night before the experiment for 6 h; 4 h after anesthesia, the light time of 8:00 am was taken as the zeitgeber time (ZT0), and then, every 8 h was taken as the recorded time (ZT8, ZT16, ZT24, ZT32 and ZT40). The RORα and Rev-erbα mRNA expressions were measured by real-time fluorescent quantitative PCR (RT-qPCR) at each time point in the cerebral cortex and hippocampus of the two groups. The cosine curves of RORα and Rev-erbα mRNA expressions were analyzed by Chronos-Fit software.
ResultsAs compared with the control group, the isoflurane anesthesia group had significantly down-regulated RORα mRNA expression in the hippocampus, significantly up-regulated RORα mRNA expression in the cerebral cortex, and significantly down-regulated Rev-erbα mRNA expression in the cerebral cortex (P<0.05). TheRORα and Rev-erbα mRNA expressions in the hippocampus and cerebral cortex of the control group showed a rhythmic cycle of about 24 h; while the circadian rhythm of RORα mRNA shifted to the right by 5.41 h, and the circadian rhythm of Rev-erbα mRNA shifted to the left by 0.89 h in the hippocampus of the isoflurane anesthesia group. The peak circadian rhythm of RORα mRNA shifted to the right by 0.35 h, and the peak circadian rhythm of Rev-erbα mRNA shifted to the left by 0.56 h in the cerebral cortex of the isoflurane anesthesia group.
ConclusionIsoflurane anesthesia can induce the changes of RORα and Rev-erbα transcription levels and circadian rhythm changes in the hippocampus and cerebral cortex of mice.
Key words:
Isoflurane; Hippocampus; Cerebral cortex; Clock gene; Gene transcription; Circadian rhythm
Contributor Information
Chen Tianyun
Department of Anesthesiology, Jinling Hospital, Medical School of Nanjing University (General Hospital of People's Liberation Army Eastern Theater Command), Nanjing 210002, China
Du Ying
Medical School of Nanjing University, Nanjing 210000, China
Fang Xin
Medical School of Nanjing University, Nanjing 210000, China
Xing Fang
Medical School of Nanjing University, Nanjing 210000, China
Ji Qing
Department of Anesthesiology, Jinling Hospital, Medical School of Nanjing University (General Hospital of People's Liberation Army Eastern Theater Command), Nanjing 210002, China
