Clinical Genetics
Association of polymorphisms of VEGF and VEGFR1 pathways-related genes and risk of pre-eclampsia
Li Li, Guo Xiangcui, Chen Beibei, Gao Zhihui, Liu Juan, Wang Qianqing
Published 2022-08-10
Cite as Chin J Med Genet, 2022, 39(8): 893-897. DOI: 10.3760/cma.j.cn511374-20210623-00534
Abstract
ObjectiveTo assess the association of single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 1 (VEGFR1) pathways-related genes and the risk of pre-eclampsia.
MethodsIn total 178 pregnant women with pre-eclampsia (case group) and 100 healthy pregnant women (control group) during the third trimester were enrolled. The SNPs of VEGF rs3025039, rs2010963 and VEGFR1 rs3812867, rs55875014 and rs722503 loci were determined by PCR-restriction fragment length polymorphism (PCR-RFLP) assay. The levels of serum VEGF and sVEGFR1 were also determined. And their association with pre-eclampsia was analyzed.
ResultsThe systolic blood pressure, diastolic blood pressure and sVEGFR1 of the case group were significantly higher than those of the control group, while the VEGF level was significantly lower than that in the control group (P<0.05). Allelic frequencies of the VEGF (rs3025039, rs2010963) and VEGFR1 (rs3812867, rs55875014, rs722503) have fit the Hardy-Weinberg equilibrium (P>0.05). The frequency of T allele of VEGF at rs3025039 locus in the case group was higher than that in the control group (P<0.05). There were significant differences in VEGF at rs3025039 locus under dominant and co-dominant models in case group (P<0.05). Compared with those with CC, the risk was higher in patients with CT or TT genotypes (P<0.05). The systolic and diastolic blood pressure and sVEGFR1 in pre-eclampsia pregnant women with CT or TT genotypes were significantly higher than those with the CC genotype, while their VEGF level was significantly lower (P<0.05). No significant difference was found in allelic frequencies of other four loci between the two groups (P>0.05).
ConclusionPolymorphisms of rs3025039 locus of VEGF gene is associated with the occurrence of pre-eclampsia. The variant at this locus may affect the activity of VEGF and influence the development of pre-eclampsia.
Key words:
Pre-eclampsia; Vascular endothelial growth factor; Vascular endothelial growth factor receptor 1; Genetic polymorphism
Contributor Information
Li Li
Department of Gynecology and Oncology, Xinxiang Central Hospital, Xinxiang, Henan 453000, China
Guo Xiangcui
Department of Gynecology and Oncology, Xinxiang Central Hospital, Xinxiang, Henan 453000, China
Chen Beibei
Department of Gynecology and Oncology, Xinxiang Central Hospital, Xinxiang, Henan 453000, China
Gao Zhihui
Department of Gynecology and Oncology, Xinxiang Central Hospital, Xinxiang, Henan 453000, China
Liu Juan
Department of Gynecology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, China
Wang Qianqing
Department of Gynecology and Oncology, Xinxiang Central Hospital, Xinxiang, Henan 453000, China
