Review
Application and research progress of cell-free DNA in the diagnosis and treatment of related disease
Lu Xinyi, Meng Qingtai, Huang Ping, Duan Yagan, Chen Zhiyuan, Xu Peng, Zhang Fengmin
Published 2024-02-19
Cite as Chin J Lab Med, 2024, 47(2): 197-204. DOI: 10.3760/cma.j.cn114452-20230729-00032
Abstract
Cell-free DNA (cfDNA) is the DNA fragment existing in human extracellular fluid. In specific physiological process (such as pregnancy) or pathological conditions (such as human malignancies), the contents of cfDNA in extracellular fluid will abnormally change. The contents and molecular characteristics of cfDNA make it have the potential as a kind of biomarker for diseases′ diagnosis. With the development of cfDNA detection technology such as sequencing and mass spectrometry, liquid biopsy based on cfDNA detection has been widely used in clinical tumor diagnosis, tumor treatment, prenatal examination, and research in autoimmune diseases. A systematic summary of the latest research progress in the development of cfDNA detection technology and the clinical application of liquid biopsy, as well as the research progress of cfDNA in the diagnosis and treatment of related diseases is summarized in this review.
Key words:
Gene test; Sequencing; Cancer; Prenatal examination; Autoimmune disease
Contributor Information
Lu Xinyi
Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Immunity and Infection, The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions, Harbin 150081, China
Meng Qingtai
Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Immunity and Infection, The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions, Harbin 150081, China
Huang Ping
Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Immunity and Infection, The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions, Harbin 150081, China
Duan Yagan
Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Immunity and Infection, The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions, Harbin 150081, China
Chen Zhiyuan
Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Immunity and Infection, The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions, Harbin 150081, China
Xu Peng
Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Immunity and Infection, The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions, Harbin 150081, China
Zhang Fengmin
Department of Microbiology, WU Lien-Teh Institute, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Immunity and Infection, The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions, Harbin 150081, China
