Gastrointestinal cancers are among the top-ranking malignancies globally, with both high incidence and mortalities. Some advanced tumor need to receive further chemotherapy, radiotherapy, targeted therapy or immunotherapy. The sensitivity of tumor cells to treatment is the key factor that determines patients' outcomes. Tumor organoids could be used to evaluate therapeutic sensitivity, because its genetic characteristics and biological behaviors are highly consistent with the original tumors. Digestive endoscopy has unique advantages in digestive malignant tumor biopsy. However, there was no consensus concerning endoscopic tissue biopsy and corresponding tumor organoid culture methods of digestive malignant tumor at present in China. Therefore, Medical-Engineering Collaborative Group, Gastroenterology Branch of Chinese Medical Association organized authoritative experts in this field to formulate this expert consensus regarding endoscopic biopsy and organoids culture methods combining evidence-based and bio-experimental evidence at home and abroad so as to standardize operational protocol in medical institutes and platforms of organoid culture. This consensus is composed of four categories including indication and morality, endoscopic biopsy, sample storage and transportation, and organoid culture with 15 statements.

Digestive system neoplasms; Organoids; Biopsy; Culture; Consensus