Clinical Genetics
Genetic analysis of a child with Generalized arterial calcification of infancy due to variant of ABCC6 gene
Zhao Chunjuan, Liu Tiantian, Liu Fang, Cui Liru, Wang Junping
Published 2024-06-10
Cite as Chin J Med Genet, 2024, 41(6): 734-740. DOI: 10.3760/cma.j.cn511374-20230525-00318
Abstract
ObjectiveTo explore the clinical manifestations and genetic basis for a rare case of Generalized arterial calcification of infancy (GACI).
MethodsA 44-day-old female infant who was treated at Baoding Hospital of Beijing Children′s Hospital Affiliated to Capital Medical University on August 26, 2022 was selected as the study subject. Clinical data of the child was collected, and Trio-whole exome sequencing (Trio-WES), whole genome copy number variation sequencing (CNV-seq) and minigene splicing assay were carried out to analyze the pathogenicity of the variants.
ResultsThe child had presented with fever and high inflammatory indicators, for which treatment with various antibiotics was ineffective. Ultrasound had revealed extensive arterial calcification and arterial wall thickening. The child was suspected for GACI with arteritis related to the primary disease. Her fever was relieved by treatment with glucocorticoid and biological agents. Trio-WES revealed that she has harbored compound heterozygous variants of the ABCC6 gene, namely c. 4404-1G>A and c. 4041+ 5G>T, for which the latter was unreported previously. Based on the guidelines from the American College of Medical Genetics and Genomics, the variants were classified as likely pathogenic (PVS1+ PM2_Supporting) and variant of unknown significance (PM2_Supporting+ PM3+ PP3), respectively. The result of CNV-seq was negative. And the minigene splicing assay has further verified that both variants can result in alternative splicing.
ConclusionFor pyrexia with unknown causes and refractory to conventional treatment, it is necessary to recommend early genetic testing to avoid missed diagnosis of GACI.
Key words:
Fever; Generalized arterial calcification; ABCC6 gene variant; Whole exome sequencing; Minigene splicing assay
Contributor Information
Zhao Chunjuan
Department of Neonatology, Baoding Hospital of Beijing Children′s Hospital Affiliated to Capital Medical University, Baoding, Hebei 071000, China
Liu Tiantian
Department of Neonatology, Baoding Hospital of Beijing Children′s Hospital Affiliated to Capital Medical University, Baoding, Hebei 071000, China
Liu Fang
Department of Neonatology, 980 Hospital of PLA Joint Logistic Support Force, Shijiazhuang, Hebei 050000, China
Cui Liru
Department of Neonatology, Baoding Hospital of Beijing Children′s Hospital Affiliated to Capital Medical University, Baoding, Hebei 071000, China
Wang Junping
Department of Function, Baoding Hospital of Beijing Children′s Hospital Affiliated to Capital Medical University, Baoding, Hebei 071000, China
