Original Article
Preparation and biosafety assessment of sustained-release drug-loading nano-micelle for the co-delivery of indocyanine green and paclitaxel
Zhifen He, Wenguang Zhao, Jing Yu, Xingyi Li, Shuai Shi
Published 2016-09-08
Cite as Chin Med, 2016, 11(9): 1404-1409. DOI: 10.3760/cma.j.issn.1673-4777.2016.09.035
Abstract
ObjectiveTo analyze the nano-micelle drug-loading system for the co-delivery of indocyanine green and paclitaxel.
MethodsThe tri-block polymer of polyethylene glycol-polycaprolactone-polyethyleneimine(PEG-PCL-g-PEI) micelle was prepared and used for drug-loading; the paclitaxel was loaded by the self-assembled hydrophobic core of PCL; the indocyanine green was adsorbed by the positive charges on micelle surface. The cytotoxicity test, in vitro drug release test and near-infrared thermal imaging were used to verify the feasibility of drug-laoding and co-delivery. The routine blood test and biochemical test were used to evaluate the biosafety of polymer carrier.
ResultsThe drug-loading system was simultaneously loaded with indocyanine green and paclitaxel through physicochemical characteristics and methoxy-polyethylene glycol(MPEG)2 000-PCL6 000-PEI2 000had the best drug-loading capacity[(9.5±3.5)wt%] and entrapment efficiency[(87.2±4.4)%]. The co-delivery system showed tumor targeting effect through thermal imaging. The polymer micelle showed good biocompatibility through routine blood test and biochemical test.
ConclusionThe tri-block polymer of MPEG-PCL-g-PEI micelle can simultaneously load indocyanine green and paclitaxel, and it can effectively achieve the co-delivery of drugs with good biocompatibility.
Key words:
Co-delivery; Indocyanine green; Paclitaxel; Nano-micelles
Contributor Information
Zhifen He
Research Group of Ophthalmological Drug Carriers, the Eye Hospital of Wenzhou Medical University, Zhejiang Province, Wenzhou 325035, China
Wenguang Zhao
Laboratory of Nano-biomaterials and Innovative Drug Carriers, Wenzhou Institute of Biomaterials and Engineering, Zhejiang Province, Wenzhou 325035, China
Jing Yu
Laboratory of Nano-biomaterials and Innovative Drug Carriers, Wenzhou Institute of Biomaterials and Engineering, Zhejiang Province, Wenzhou 325035, China
Xingyi Li
Research Group of Ophthalmological Drug Carriers, the Eye Hospital of Wenzhou Medical University, Zhejiang Province, Wenzhou 325035, China
Shuai Shi
Research Group of Ophthalmological Drug Carriers, the Eye Hospital of Wenzhou Medical University, Zhejiang Province, Wenzhou 325035, China
