Original Article
Expression of microRNA-141 in bladder cancer and its correlation with tumor grade
Wang Zhenxing, Pei Liang, Li Shuangping, Hao Zhixuan
Published 2020-04-25
Cite as China Clin Pract Med, 2020, 11(2): 15-19. DOI: 10.3760/cma.j.cn115570-20200117-00083
Abstract
ObjectiveTo investigate the expression of microRNA-141(miR-141) in bladder cancer and its correlation with tumor grade.
MethodsA total of 80 patients with bladder cancer admitted to the department of Urology of the Second Hospital of Shanxi Medical University from March 2012 to March 2014 were selected, including 67 males and 13 females, aged(68.23±3.57)years old, ranging from 61 to 83 years old.The relative expression of miR-141 mRNA in bladder cancer tissues and adjacent normal tissues were detected by real-time polymerase chain reaction(RT-PCR). Correlation of miR-141 expression with clinicopathological characteristics and prognosis of bladder cancer patients was analyzed.The overall survival rate(OS)and progression-free survival rate(PFS)were compared between patients with low miR-141 expression and high miR-141 expression.
ResultsThe relative expression of miR-141 mRNA in bladder cancer tissues was significantly higher than that in normal adjacent tissues [(4.93±0.88)versus(0.83±0.11), P<0.05]; The relative expression of miR-141 mRNA had no significant difference among bladder cancer patients with different genders and ages(P>0.05). The relative expression of miR-141 mRNA had significant difference among bladder cancer patients with different tumor grades, tumor stages, muscular invasion and tumor metastasis(P<0.05). The 3-year survival rate of bladder cancer patients with different tumor grades, tumor stages, tumor metastasis, and miR-141 expression showed significant difference(P<0.05). Tumor grade, tumor stage, tumor metastasis, and miR-141 expression were independent risk factors that affect the prognosis of bladder cancer patients(P<0.05). OS and PFS of miR-141 low expression patients were significantly higher than those of miR-141 high expression patients, with statistic difference[70.97%(22/31)versus 20.41%(10/49); 64.52%(20/31)versus 24.49%(12/49),P<0.05].
ConclusionThe miR-141 is highly expressed in bladder cancer patients, and is closely related to tumor grade, tumor stage, tumor metastasis and prognosis of bladder cancer patients, which can provide a reference for early diagnosis and prognosis of bladder cancer patients.
Key words:
MicroRNA-141; Bladder cancer; Tumor classification; Relative expression
Contributor Information
Wang Zhenxing
Department of Urology, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Pei Liang
Department of Urology, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Li Shuangping
Department of Urology, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Hao Zhixuan
Department of Urology, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
