Original Article
Effect of bioaerosol exposure via respiratory tract quantitative inhalation on inflammatory factors secretion
Zhang Jianping, Chen Shuanghong, Ren Xiaomeng, Zhou Hongyuan, Tang Ying
Published 2022-08-28
Cite as Chin J Health Manage, 2022, 29(4): 528-534. DOI: 10.3760/cma.j.cn311847-20220130-00037
Abstract
ObjectiveTo establish a bioaerosol exposure model by quantitative atomized inhalation via respiratory tract，and study the sensitive indexes for early evaluation of inflammatory stress caused by exposure damage.
MethodsPseudomonas aeruginosa（ATCC15692）was used to simulated as the aerosol. Six-week-old SD rats were chose as the exposed subjects and they were randomly divided into four groups. Control group（24 rats）was exposed to phosphate-buffered saline（PBS）aerosol；low-dose group（28 rats）was exposed to 1.6×104 cfu/ml of ATCC15692 aerosol；medium-dose group（40 rats）was exposed to 8.0×104 cfu/ml of ATCC15692 aerosol；high-dose group（48 rats）was exposed to 1.6×105 cfu/ml of ATCC15692 aerosol. Masson staining was used to exam the lung tissue elastic fibers，and ELISA was used to detect the secretion levels of serum C-reactive protein（CRP），interleukin 6（IL-6），interleukin 10（IL-10），procalcitonin（PCT），serum amyloid A（SAA），tumor necrosis factor alpha（TNF-α），and clara cell protein（CC16）.
ResultsAfter contamination，compared with the control group，the levels of CRP，IL-6，PCT，SAA，TNF-α，and CC16 of the experimental groups were all increased，and those in the medium-dose group increased significantly（t=-4.71–-14.88，P<0.01；t=-9.28–-28.29，P<0.01；t=-6.04–-12.97，P<0.01；t=-8.93–-19.12，P<0.01；t=-9.65–-148.76，P<0.01；t=-8.91–-11.90，P<0.01）. Compared with the control group，after contamination，the IL-10 levels of the experimental groups decreased significantly at the detection time points of 24 h，48 h，72 h，especially those of the medium-dose group（t=-6.74–28.86，P<0.01），and the IL-10 levels increased significantly at 144 h（t=-4.06–-6.12，P≤0.01）. Within the same experimental dose group at different time points，the levels of CC16 showed a downward trend. The levels of CC16 in the low-dose group decreased significantly from 24 h to 48 h（t=2.78，P=0.05），and that in the medium-dose group decreased significantly at 144 h（t=4.41，P=0.01）. The levels of TNF-α in the medium-dose group decreased significantly after 48 h（t=4.56，P=0.01）. The levels of IL-6 in two experimental dose groups showed a trend of increasing within 24–72 h，but decreased significantly at 144 h（t=5.91–28.45，P<0.01）. The levels of CRP，PCT，and SAA in the low-dose group kept increasing throughout the measurement time course，while those in the medium-dose group increased within 72 h and then decreased significantly at 144 h（t=2.10–22.27，P<0.01）.
ConclusionBioaerosol inhalation exposure can effectively induce the inflammatory response of the respiratory system. The secretion levels of serum CRP，IL-6，IL-10，PCT，SAA，TNF-α，and CC16 change significantly with time，which may be used as candidate sensitive indicators for early evaluation of bioaerosol inhalation exposure damage.
Key words:
Bioaerosol; Quantitative inhalation via respiratory tract; Inflammatory stress; Inflammatory factors
Contributor Information
Zhang Jianping
Naval Medical Center，Naval Medical University，Shanghai 200433，China
Chen Shuanghong
Naval Medical Center，Naval Medical University，Shanghai 200433，China
Ren Xiaomeng
Naval Medical Center，Naval Medical University，Shanghai 200433，China
Zhou Hongyuan
Naval Medical Center，Naval Medical University，Shanghai 200433，China
Tang Ying
Naval Medical Center，Naval Medical University，Shanghai 200433，China
