Original Article
Establishment of total parenteral nutrition model and study on intestinal barrier function in newborn Bama mini-pig
Liu Yang, Xiao Yongtao, Chen Shanshan, Tian Xinbei, Wang Weipeng, Wu Wenjie, Zhang Tian, Wang Ying, Cai Wei
Published 2020-08-30
Cite as Chin J Clin Nutr, 2020, 28(4): 238-244. DOI: 10.3760/cma.j.cn115822-20200626-00157
Abstract
ObjectiveTo better simulate parenteral nutrition-associated complications in pediatrics, establishment of enteral and parenteral nutrition-related models were planned with newborn Bama mini-pig.
MethodsEighteen newborn Bama mini-pigs were divided into enteral nutrition (EN) groups and total parenteral nutrition (TPN) groups. EN groups were further divided into EN-1W (n=3) and EN-2W (n=3); and TPN groups were divided into TPN-1W (n=6) and TPN-2W (n=6). The weight and histomorphological changes of newborn Bama mini-pigs were observed. Liver function and intestinal barrier function were also detected and analyzed.
ResultsDuring modeling, all Bama mini-pigs in TPN groups survived, the related complications of TPN-2W mini-pigs were more obvious than that of TPN-1W mini-pigs. Compared with EN-2W group, the weight of TPN-2W group decreased significantly (P<0.05). Serum total bilirubin, direct bilirubin, and bile acid levels in TPN-2W group were significantly higher than those in EN-2W group (P<0.05). The hepatic histomorphology of the TPN groups showed scattered portal inflammatory infiltration and mild hepatic steatosis. Compared with EN-2W group, the intestinal villi of the TPN-2W mini-pigs were atrophic and deformed, with significantly decreased villus length (P<0.05), and crypt depth (P<0.01), and significantly increased intestinal permeability (P<0.05).
ConclusionAn experimental model of total parenteral nutrition can be established using the newborn Bama mini-pigs, which can well simulate the parenteral nutrition-associated liver damage and intestinal barrier injury in infants and young children, thus providing a better animal model to study the pathogenesis of clinical parenteral nutrition-associated complications in pediatrics.
Key words:
Total parenteral nutrition; Piglet; Animal models; Parenteral nutrition-associated liver disease
Contributor Information
Liu Yang
Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
Xiao Yongtao
Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
Chen Shanshan
Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai 200092, China
Tian Xinbei
Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai 200092, China
Wang Weipeng
Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai 200092, China
Wu Wenjie
Department of Pediatric Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
Zhang Tian
Department of Pediatric Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
Wang Ying
Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
Cai Wei
Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China
