To analyze the long-term stability of domestic keratoprosthesis (Kpro) after its application in rabbits' corneas.

In this experimental study, domestic permanent modified keratoprosthesis (Moroz Kpro) were implanted in the right eyes of 10 rabbits. The left eyes served as control. Surgery involved two steps: Successively implantation of titanium frame first and then the implantation of optical cylinder. In the first stage, the third eyelid (cartilage, equivalent to) was removed and used as reinforcement of corneal surface and then autologous conjunctival was covered at the surface (Stage 1); secondly, an optical cylinder was implanted 3 months after the first stage (Stage 2). Postoperative observation follow up for 6 months. Postoperative antibiotic eye drops were given to prevent infection. Finally the corneas were observed histopathological, compared with the left eyes.

One rabbit died because of intraoperative anesthesia during the operation of stage 2. The other 9 rabbits survived till the end of 6-month follow up. These Kpros retained till the end without severe complications, such as corneal melting, aqueous humor leakage, high intraocular pressure or infection etc. Biopsy specimen showed: The cartilages were in place; the frame and the surrounding tissue combined closely; there was a more active proliferation of fibrous tissue around the Kpro, and the keratitis reactions were very slight. The left eyes were normal.

Domestic keratoprosthesis has a good biocompatibility and stability after the application in the cornea of rabbits. The application of cartilage and autologous conjunctival play an important role in the success of keratoprosthesis retention.

Corneal transplantation; Materials testing; Keratoprosthesis; Cartilage histological