Experimental Sciences
Screening of optimal ultrasound parameters after intravitreal injection of microbubble in normal rabbit eyes
Shen Xiaoli, Li Qiang, Huang Lina, Zhao Jun, Fan Ning
Published 2016-11-10
Cite as Chin J Exp Ophthalmol, 2016,34(11): 983-988. DOI: 10.3760/cma.j.issn.2095-0160.2016.11.005
Abstract
BackgroundUltrasound microbubbles play important effects on delivery of drug or gene, which can significantly improve the transfection efficiency of drug or gene after ultrasonic irradiation.However, different ultrasonic irradiation parameters have different biological effects on different cells.
ObjectivesThis study was to investigate the effects of ultrasound microbubble on normal rabbit retina in different ultrasound intensities and irradiation time and provide the experimental basis for the treating study of fundus diseases with ultrasound microbubble.
MethodsEighteen clean New Zealand albino rabbits were randomized into blank control group, simple ultrasound radiation group, simple intraocular injection group and intraocular injection+ low, moderate and high intensity ultrasound groups.No intervention was performed in the blank control group.Only 2.0 W/cm2 ultrasound radiated for 60 seconds or 0.1 ml microbubbles were injected in vitreous cavity respectively in the simple ultrasound radiation group or simple intraocular injection group.The ultrasound with the intensity of 0.5, 1.0 and 2.0 W/cm2 radiated the eyes with intraocular injection of microbubbles for 60 seconds in the intraocular injection+ low, moderate and high intensity ultrasound groups, respectively.The photography of ocular anterior segment, direct ophthalmoscopy and color fundus photography were performed before operation, immediately after operation and 1 day, 7 days after operation.The retinas were isolated in the seventh day and hematoxylin & eosin staining was carried out for the evaluation of retinal morphology.The ultrastructure of the retinas was examined by transmission electron microscope.This study protocol was approved by Ethic Committee of Experimental Animals Use and Care in Shenzhen Eye Hospital.
ResultsThere were not abnomal findings by slit lamp microscope and ophthalmoscope in the eyes of various groups at different time points.In the intraocular injection+ moderate and high intensity ultrasound groups, the loosening of retinal layers, disorder of photoreceptors, thinning of inner and outer nuclear layers as well as the decrease of retinal ganglion cells (RGCs) were exhibited under the optical microscope, and mitochondrial swelling of photoreceptors, noneven of chromatin in nucleus and losing of RGCs were found under the transmission electron microscope.However, the retinal morphology and ultrastructure were normal in the blank control group, simple ultrasound radiation group, simple intraocular injection group and intraocular injection+ low intensity ultrasound group.
ConclusionsUltrasound irradiation in the intensity 0.5 W/cm2 for 60 seconds is safe and suitable for retina in the eyes with intraocular injection of microbubble.This result provides an experimental basis for the management of fundus diseases by ultrasound microbubble.
Key words:
Retina; Ultrasonics; Microbubbles; Sonication; Intravitreal injection; Ultrasound parameter; Rabbits
Contributor Information
Shen Xiaoli
Shenzhen Key Laboratory, Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Li Qiang
Department of Ophthalmology, Shenzhen People's Hospital, Jinan University, Shenzhen 518000, China
Huang Lina
Shenzhen Key Laboratory, Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Zhao Jun
Shenzhen Key Laboratory, Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
Fan Ning
Shenzhen Key Laboratory, Shenzhen Eye Institute, Shenzhen Eye Hospital, Jinan University, Shenzhen 518040, China
